Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FlexiTube siRNA Premix works with minimal siRNA concentrations. To decrease the final siRNA concentration, simply use smaller volumes of FlexiTube siRNA Premix for transfection. For example, using only half of the volume given in the protocol will lead to a final siRNA concentration of 12.5 nM. Depending on the cell type, final siRNA concentrations of down to 1 nM can lead to sufficient gene silencing.