Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?

Even though the blocking of MAPK1 expression potentially has secondary effects on the cell cycle, it has proven very successful for use as a knock-down control in siRNA transfection experiments. We have monitored gene knock-down for 5 days using MAPK1 without any visible negative effects on cell survival.

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