What is the recipe for SOC medium?
The components of the SOC medium are:
- 0.5% Yeast Extract
- 2% Tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 10 mM MgCl2
- 10 mM MgSO4
- 20 mM Glucose*
*Note: add Glucose after autoclaving the solution with the remaining ingredients, and letting it cool down. Sterilize the final solution by passing it through a 0.2 µm filter.
SOC medium can be stored at room temperature and is stable for several years.