FAQ ID -315

How can QIAGEN Protease and Proteinase K be inactivated?

QIAGEN Protease is inactivated by incubation at 70°C for 15 minutes.

To our knowledge, Proteinase K cannot be completely heat-inactivated. Even when incubating at 95°C for 10 minutes, some enzymatic activity remains. This will not negatively affect the QIAamp Procedure, since the enzyme will be efficiently removed by the wash steps in the protocols.

Can’t find what you are looking for?

Browse the FAQ base with our FAQ search.