How do I resuspend my FlexiPlate siRNAs?
FlexiPlate siRNAs are preannealed and dried down from a 10 µM solution in buffer. They should be resuspended in sterile RNase-free water only (you do not need siRNA buffer to resuspend the siRNAs).
To achieve a final siRNA stock concentration of 10 µM, resuspend:
- 0.1 nmol siRNA in 10 µl
- 0.25 nmol siRNA in 25 µl
- 1 nmol siRNA in 100 µl
Mix gently and cover the plate with new sealing film. Allow siRNAs to dissolve for 30 minutes at 4°C with occasional shaking or gentle vortexing.