Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays?
The performance of our RT² qPCR Primer Assays have been tested, and are guaranteed with, our RT² SYBR Green Mastermixes only. Our primer design algorithm accounts for our master mix buffer system parameters such as ionic strength and magnesium chloride concentration. We have not tested our Primer Assays with other sources of master mix and their buffer system parameters. We only guarantee that our primers will perform optimally with our master mixes. We do not guarantee optimal performance with other sources of master mix, without requiring further optimization studies by the end-user.