Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

Most antibodies used in immunohistochemical assays were developed for use with formalin-fixed tissue and include steps for unmasking epitopes. When working with PAXgene Tissue treated specimens, test for each antibody to determine if it is necessary to perform antigen-retrieval steps. In addition, it is often necessary to optimize antigen-retrieval steps and/or to adjust antibody concentration in PFPE tissue to achieve optimal staining intensities (see technical note: “Effect of epitope retrieval conditions on immunohistochemical staining of PFPE tonsil tissue with anti-human Ki-67 antigen (clone MIB-1)”). Examples for IHC staining of adjacent tissue sections from human fixed with neutral-buffered formalin or with PAXgene Tissue reagents are provided in the Tissue Atlas.


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