How does QIAGEN control the quality of the RT² qPCR Primer Assays?
Each RT² qPCR Primer Assay is validated with both a real-time and conventional PCR quality control assay. These assays are carried out using a single source of genomic DNA. In order to pass QC, each primer assay must generate a single band of the correct predicted size by agarose gel electrophoresis and a single peak in the real-time dissociation curve without the appearance of primer dimers. The amplification efficiencies (DART method) and sensitivities of each primer set are also validated.