3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits?

In-house experiments using the standard RNeasy procedure for purifying RNA from different tissues showed that RNA yields were comparable when using ethanol, methanol or isopropanol. However, preparations with methanol and isopropanol resulted in much higher contamination with genomic DNA, despite performing on-column DNase digests with the RNase-Free DNase Set. Therefore, we do not recommend the use of isopropanol or methanol for RNeasy preparations. To ensure highest possible RNA quality, only ethanol should be used.


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