Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples?

Formalin-fixed, ethanol-preserved samples that are not embedded in paraffin can be processed with the REPLI-g FFPE Kit by leaving out step 4 and 5 of the REPLI-g FFPE protocol. A mix containing FFPE Lysis Solution* and 2 µl Proteinase K can be added to the tissue section at step 3, after the section has been removed from its preservative solution. Proceed with the protocol at step 7: Incubate for 60 min at 60°C and then for a further 10 min at 95°C.

*Typically, 100 µl FFPE Lysis Solution are used. However, a smaller volume can be used for trimmed tissue sections (see protocol step 3 in the REPLI-g FFPE Handbook for more information).



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