At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?

Temperature is the most important factor in the NexTal Cubic Phase experiment. In fact, in meso phase crystallization is very temperature sensitive. The working range is between 20 and 24°C, however 22°C is the optimal temperature. Below these temperatures, MO crystal-like structures will form and will be very difficult to distinguish from protein crystals. Also the chance of obtaining protein crystals will be very low. Lower temperatures can be used if the lipidic phase contains, for example, monoolein + 8% cholesterol. In this case, temperatures as low as 18°C are used.

Note: All GPCR crystals reported in literature so far were crystallized at 20°C – 24°C


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