FAQ ID -2687

How can I determine whether amplification occurs from mRNA-derived cDNA or from genomic DNA contamination?

The most rigorous method to detect genomic DNA contamination, particularly with the RT² qPCR Primer Assays, is to perform a No Reverse Transcriptase (NRT) control. The PCR will have no cDNA template derived from mRNA, and any detectable product could only have been derived from genomic DNA contamination.

Can’t find what you are looking for?

Browse the FAQ base with our FAQ search.