What are the advantages of RT-PCR based somatic mutation PCR arrays and assays compared to other platforms?
The main advantages are qPCR-based superior detection sensitivity and straightforward data analysis procedure. Additional major advantages over other currently available mutation detection platforms/methods are: (1) The workflow is very simple, involving only one setup step. No multi-step handling is involved, and hands-on time is less than any other method available. (2) Reactions involved are all closed-tube reactions avoiding sample contamination. (3) The DNA sample input is low. (4) The hardware involved in analysis using the mutation detection arrays and assays is highly accessible, enabling such analysis for any laboratory with access to real-time PCR instruments.