Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?

Yes. Do not increase the activation time for HotStarTaq Plus DNA Polymerase beyond the recommended 5 minutes, as PCR product yield may be reduced and less specific amplification may result. Please follow the instructions in the HotStarTaq Plus PCR Handbook closely, and use the cycling parameters presented in the handbook to ensure optimal results.



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