Can the nuclei and cell debris fraction generated in the QProteome Mitochondria isolation procedure be used for further fractionation?
We have not tested this application. We would suggest to split the sample, and perform different fractionation procedures separately on the two sample portions.
If splitting the sample is not feasible, then it should theoretically be possible to use the pellet generated at step 7 of the QProteome Mitochondria Isolation Kit protocol (containing cell debris, nuclei and unbroken cells) as starting material for the Qproteome Nuclear Protein Kit.
Please note that this application will have to be tested and optimized by the enduser.