What type of samples can I use for the KRAS PCR and EGFR kits?

We recommend the use of samples obtained by surgical resection. The reason for this is to ensure a sufficient quantity of DNA, and also so a non-homogenous tumour is accurately represented in the sample.   FFPE samples are often fragmented or contain inhibitors. Check for inhibition by examining the Internal Control. Fragmentation can prevent amplification despite the amplicon size of the reactions in the kit being optimized to work with small fragments. Samples obtained by Fine Needle Aspiration (FNA) may not be sufficient enough.

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