Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast?

The PhosphoProtein Purification Kit is designed for the isolation of phosphoproteins from mammalian cells.

However, there is a customer reference available mentioning the successful enrichment of phosphoproteins from yeast as starting material using this kit, and it may therefore be worth a try. The reference is "Rapid enrichment and analysis of yeast phosphoproteins using affinity chromatography, 2D-PAGE and peptide mass fingerprinting" by Makrantoni et al., Yeast, 2005, 22(5): 401-14.


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