How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR?

EpiTect ChIP qPCR Assays are designed to detect specific genomic DNA sequences within a 30 kb promoter region surrounding every transcription start site (TSS) in the RefSeq database. They are optimized and extensively validated with SBGR master mix using both genomic DNA and real ChIP samples. ChIP PCR Primers meet all the essential requirements for accurate and reliable delta-delta Ct calculations for absolute fractional enrichment of a target nuclear factor at a specific promoter site as well as relative fold-change differences across multiple samples. For details on these calculations and on interpreting the results, see our EpiTect ChIP qPCR Assay Data Analysis Template.

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