The QIAseq FastSelect works by prevented cDNA synthesis of unwanted RNAs during library prep.
To enable this, prior to RNA heat fragmentation, the FastSelect reagent is directly combined with total RNA (1 ng – 1 µg) and the library-prep–specific buffers. Heat fragmentation is then performed, and the reaction temperature is gradually cooled to room temperature. Following this, the remaining library-prep–specific steps are followed. There is no need to perform any type of enrichment on the total RNA samples.