Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays?
Absolutely not. Due to the GC-rich nature of CpG islands, EpiTect Methyl qPCR Primers require a very specific combination of PCR temperature cycle and chemistry to obtain reliable and accurate DNA methylation analysis. Without the correct PCR cycling program, your DNA methylation analysis will fail due to the non-specific amplification during PCR. Do double check the manual for the appropriate PCR cycle for EpiTect Methyl qPCR DNA methylation analysis for you instrument model.