What is the composition of buffer QF?
Buffer QF is the elution buffer used in QIAGEN Plasmid Kits for plasmid purification and in QIAGEN Blood & Cell culture kits.
The composition of Buffer QF is:
- 1.25 M NaCl
- 50 mM Tris-Cl, pH 8.5
- 15% isopropanol (v/v)
To make 1 liter of solution, dissolve 73.05 g NaCl and 6.06 g Tris base in 800 ml distilled water. Adjust the pH to 8.5 with HCl. Add 150 ml pure isopropanol. Adjust the volume to 1 liter with distilled water. Store at 15–25°C