What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?

  • The gDNA removal should be performed at room temperature from 15-30°C.
  • Perform the gDNA removal step for 5 minutes. However, the incubation can be prolonged to 15 minutes. An incubation longer than 15 minutes is not recommended.
  • The gDNA elimination reaction is efficiently stopped by adding the QuantiFast Mix 2.





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