Microbiome WGS SeqSets

• Integrated solutions for DNA extraction, whole genome metagenomics and bioinformatic analysis
• Efficient workflow from DNA isolation to data analysis
• Compatibility with diverse microbiome research applications
• Includes access to the Microbial Analysis Portal for advanced taxonomic and antimicrobial resistance (AMR) analysis

The QIAamp PowerFecal Pro DNA WGS SeqSets and DNeasy PowerSoil Pro WGS SeqSets are comprehensive all-in-one solutions for microbiome research. These kits offer a streamlined process from nucleic acid extraction to whole genome metagenomics using the QIAseq FX DNA Library Prep Kit and easy-to-use bioinformatic analysis via the Microbial Analysis Portal.

These kits have been specifically designed to maximize the efficiency and reproducibility of microbiome research workflows, starting from extraction of high-quality DNA from fecal (QIAamp PowerFecal Pro DNA) or soil (DNeasy PowerSoil Pro DNA) samples, through comprehensive whole genome metagenomics and analysis. The bundled Microbial Analysis Portal provides powerful capabilities for taxonomic identification of microbes and antimicrobial resistance (AMR) analysis, making these kits a complete sample-to-insight solution for microbiome research.

Considering using these kits for the first time? Request a trial kit for the QIAamp PowerFecal PRO DNA WGS SeqSet the DNeasy PowerSoil Pro WGS SeqSet . 

Want to try out the Microbial Analysis Portal? Try out the demo here.      

For a specific quote for your research project or to discuss your project with our specialist team, contact us.

The QIAamp PowerFecal Pro DNA WGS SeqSets and DNeasy PowerSoil Pro WGS SeqSets kits are designed to obtain high-quality DNA, minimize bias and maximize the diversity of the extracted DNA, providing a representative view of the microbiome in the sample. These kits enable precise identification and characterization of the microbial species present in soil or fecal samples.

Unbiased and high-yield isolation of microbial genomic DNA 

DNeasy PowerSoil Pro DNA and QIAamp PowerFecal Pro DNA Kits isolate high yields of DNA from soil and stool/gut samples respectively. 

Both kits identify a greater bacterial diversity compared to other commercially available kits (see figures “Increased bacterial OTUs with the new DNeasy PowerSoil Pro Kit). ” and “Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit. ”)

Fast and efficient library preparation with low GC bias 

The QIAseq FX DNA Library Kit takes you from 20 pg – 1 μg genomic DNA to sequencer-ready, amplified libraries in just 2.5 hours. It’s faster and easier to automate than mechanical shearing but still generates the high-quality libraries you need for whole genome sequencing of any organism  (see figures “Consistent performance from any input” and “Workflow”).   The workflow reliably generates reproducible DNA fragments of customizable sizes. (see figure “High Reproducibility”)

The QIAseq FX DNA Library Kit performs library preparations with minimal bias by using sequence-independent enzymes for fragmentation – delivering results comparable to mechanical shearing. (see figure“Minimal GC bias compared to other enzymatic methods”)

 
Comprehensive bioinformatic analysis with Microbial Analysis Portal

The Microbial Analysis Portal provides an intuitive, easy-to-use web-based platform allowing researchers that are not bioinformatics experts to analyze the resulting data. You can perform taxonomic identification of microbes, providing a detailed breakdown of the microbial species present in the sample. Additionally, the platform supports AMR analysis, enabling the identification of antibiotic resistance genes and contributing to our understanding of antibiotic resistance spread and evolution. Publication-ready data and figures are easily and quickly generated.

The QIAamp PowerFecal Pro DNA WGS SeqSets and DNeasy PowerSoil Pro WGS SeqSets provide a unified solution for microbiome research. The process begins with the extraction of DNA from the sample using the appropriate kit. The extracted DNA is then subjected to whole genome metagenomic analysis via the QIAseq FX DNA Library Prep Kit, generating a comprehensive dataset that includes all genomic DNA in the sample. This data is then analyzed using Microbial Analysis Portal, which provides detailed insights into the taxonomic composition of the sample and the antibiotic resistance genes present. 

Sample extraction

Samples processed with the QIAamp PowerFecal Pro DNA and DNeasy PowerSoil Pro Kits are added to a bead-beating tube. Rapid and thorough homogenization occurs using mechanical and chemical methods. Once cells are lysed, our patent-pending Inhibitor Removal Technology (IRT) is used to remove inhibitors. Total genomic DNA is captured on a silica membrane in a spin-column format. DNA is then washed and eluted, ready for downstream library preparation.

Library preparation

QIAGEN’s QIAseq FX technology incorporates enzymatic DNA fragmentation into a streamlined, optimized protocol that combines fragmentation and adapter ligation in one reaction, saving time and preventing errors. The QIAseq FX kit contains a novel nuclease formulation that digests double-stranded DNA (dsDNA) in a random fashion without sequence preference, generating sequencing libraries with minimal bias. The fragment size can be changed simply by changing the incubation time of the dsDNA with the nuclease, enabling different sequencing applications. Optimized enzyme and buffer compositions ensure high sequencing library yield. Streamlined library construction protocols also enable straightforward automation of library prep on different liquid-handling platforms.

The QIAGEN QIAseq FX DNA Library Kit provides a fast, fully enzymatic procedure, from DNA fragmentation to a next-generation sequencing library, with no cleanup steps until after adapters have been ligated to the sample DNA. 

The QIAseq Normalizer workflow follows a simple bind–wash–elute routine, which is identical for all compatible types of libraries. Normalized libraries are dsDNA and have a common concentration of approximately 4 nmol/L, which allows them to be directly used for pooling and sequencing.

Analysis

After sequencing, samples undergo comprehensive analysis via the Microbial Analysis Portal for taxonomic abundance, differential abundance and AMR-gene abundance. 

The QIAamp PowerFecal Pro DNA WGS SeqSets and DNeasy PowerSoil Pro WGS SeqSets are designed for diverse microbiome research applications, including studies of gut health, soil microbiology, antibiotic resistance and many others. The comprehensive nature of these kits makes them highly versatile tools for any researcher studying the microbiome.