✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
QIAseq xHYB Pro Capture Frame
Cat no. / ID. 334141
Reusable 96-well plate accessory designed to securely hold individual xHYB Pro Hybridization Reagent tubes for stable plate-based handling. Recommended for reliable workflow execution, especially during heated on-cycler steps.
PlateKit
QIAseq xHYB Pro Capture Frame
QIAseq xHYB Pro Capture Kit (24)
QIAseq xHYB Pro Capture Kit (96)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
The QIAseq xHYB Pro Capture Kits is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Single-day workflow with hybridization in as little as 5 minutes
- Less hands-on time and workflow complexity with ready-to-use reagents and streamlined target enrichment
- Exceptional coverage uniformity across challenging genomic regions
- Automation-ready target enrichment for high-throughput sequencing workflows
Product Details
QIAseq xHYB Pro Capture Kits transform hybrid capture from an overnight process into a streamlined, single-day workflow for targeted sequencing applications including comprehensive genomic profiling (CGP), exome, and custom panels.
Its pre-dried, pre-dispensed hybridization chemistry eliminates dry-down and SpeedVac concentration steps while enabling hybridization in as little as 5 minutes, reducing hands-on time and increasing laboratory throughput. Compatible with indexed DNA- and RNA-derived libraries, QIAseq xHYB Pro integrates seamlessly with the QIAseq ecosystem to deliver an automation-ready sample-to-sequencer workflow for modern genomics laboratories.
Performance
- Coverage uniformity across complex target regions
- Minimizes coverage dropouts in high GC-content and challenging genomic regions
- High sensitivity for SNVs, indels, CNVs, and genomic signatures
- Robust performance for CGP and HRD research applications
- Consistent enrichment performance from 5-minute to overnight hybridization
- Performance evaluated on Illumina and Element Biosciences sequencing workflows
Principle
QIAseq xHYB Pro Capture Kits leverage hybrid capture technology to selectively enrich genomic regions of interest from indexed DNA- and RNA-derived libraries. Biotinylated double-stranded DNA probes hybridize to target sequences, while blocking oligos suppress nonspecific interactions and improve capture efficiency.
Unlike conventional hybrid capture workflows that require lengthy incubations and extensive reagent preparation, QIAseq xHYB Pro Kits combine optimized hybridization chemistry with a pre-dispensed reagent format to simplify setup and dramatically shorten hybridization times. Following hybridization, captured targets are isolated using streptavidin-coated magnetic beads, washed to remove non-target fragments, and amplified for downstream sequencing.
This streamlined approach enables rapid, high-throughput target enrichment while reducing workflow complexity and supporting automation-ready implementation.
Procedure
The QIAseq xHYB Pro workflow is designed to minimize manual intervention and accelerate target enrichment. Indexed DNA- or RNA-derived libraries are pooled and combined with QIAseq xHYB probe panels and the xHYB Pro Hybridization Reagent. Hybridization can be completed in as little as 5 minutes, eliminating one of the largest bottlenecks in traditional target enrichment workflows.
Following hybridization, target-probe complexes are captured using streptavidin magnetic beads, washed to remove non-target fragments, and amplified to generate sequencing-ready libraries. The simplified workflow eliminates dry-down steps, reduces hands-on time, and enables a streamlined path from library preparation to sequencing within a single working day.
| Applications | Comprehensive Genomic Profiling (CGP), HRD analysis, exome sequencing, targeted DNA sequencing |
| Input Material | Indexed DNA- or RNA-derived sequencing libraries with Illumina®-compatible adapters |
| Library Multiplexing | Up to 8 indexed libraries per hybridization |
| Compatible Library Preparation Kits | QIAseq FX DNA Library Kit |
| QIAseq Ultralow Input Library Kit | |
| QIAseq Multimodal DNA Library Kit and other workflows that generate Illumina®-compatible indexed sequencing libraries |
|
| Compatible Panels | QIAseq xHYB CGP Panels |
| QIAseq xHYB HRD Panel | |
| QIAseq Human Exome | |
| QIAseq xHYB Actionable Exome Panel | |
| QIAseq xHYB Custom Human Panels other compatible hybrid-capture probe panels utilizing 120 nt biotinylated DNA capture probes | |
| Normalization Compatibility | QIAseq Normalizer Kits for quantification-free normalization and pooling |
| Sequencing Platforms | Compatible with standard Illumina® and Element Biosciences sequencing workflows |
| Automation Compatible | Yes |
| Format | 24-sample and 96-sample kit configurations |
| Intended Use | Research Use Only (RUO) |
Applications
- Targeted next-generation sequencing of human DNA or RNA-derived libraries
- Comprehensive genomic profiling (CGP) for cancer research
- Homologous recombination deficiency (HRD) research workflows
- Whole-exome and actionable exome sequencing for research applications
- Rare disease and inherited disease research
Software
Sequencing data generated from compatible QIAseq xHYB workflows can be analyzed using QIAGEN CLC Genomics Workbench and related QIAGEN Digital Insights solutions, depending on the application and panel configuration. Where applicable, results can be seamlessly transferred to the appropriate QIAGEN interpretation solution for downstream analysis and interpretation.