The miRCURY LNA miRNA ISH Optimization Kit (FFPE) is a great tool to help you get started with or to optimize miRNA in situ
hybridizations (ISH) on formalin-fixed paraffin embedded (FFPE) tissue samples.
Based on the highly successful double (5’ and 3’) DIG-labeled miRCURY LNA miRNA Detection Probes, the kits provide the sensitivity and specificity needed to detect miRNAs (see figure Principle of the miRCURY LNA miRNA ISH Optimization Kit
). In addition, the reagents included have been specifically adapted for use with LNA-enhanced
probes in FFPE tissue sections.
Eight different miRCURY LNA miRNA ISH Optimization Kits are available. Each kit comes with positive and negative control probes, hybridization buffer and Proteinase K. In addition, a unique, tissue-specific miRCURY LNA miRNA Detection Probe is included in each kit.
Each kit includes:
- Unique miRNA LNA probe (double DIG-labeled, kit specific)
- Scrambled LNA probe (double DIG-labeled, negative control)
- U6 LNA probe (5’ DIG-labeled, positive control)
- Hybridization buffer (2x, formamide-free)
- Proteinase K
The unique miRNA LNA probes have been validated in a variety of tissues and are therefore ideal for optimizing ISH experimental settings. For recommendations on which kit to choose based on tissue type, refer to the figure miRCURY LNA miRNA ISH Kit chooser