Qproteome Cell Compartment Kit
For fractionation of proteins according to cellular location
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The Qproteome Cell Compartment Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Use of the Qproteome Cell Compartment Kit allows simple and effective cell compartment-specific localization of marker proteins from isolated cells (see figure "Specific Separation of Marker Proteins" and to "Localization of Proteins in Cells Under Different Growth Conditions") or from tissue samples (see figure "Localization of Proteins in Tissue Samples").
Principle
The kit contains 4 Extraction Buffers which enable the sequential isolation of proteins associated with the cytosol, membranes, nucleus, and cytoskeleton from cell lysates or tissues.
Procedure
The 4 Extraction Buffers are added sequentially to a cell pellet and the respective fractions are isolated by centrifugation (see flowchart). In an alternative protocol for tissues, samples are simultaneously homogenized and disrupted using the TissueRuptor before being processed as for the cell pellet.
Applications
Subcellular fractionation of proteins can be used for the enrichment of low-abundance species; to define the subcellular localization of enzymes, regulatory, and structural proteins; and for monitoring of compartmental redistribution of biomolecules under basal and stimulated conditions. Specifications
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