Knowledge & Support
Mortazavi, A., Williams, B.A., McCue, K., et al. (2008) Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat. Methods.
Marioni, J.C, Mason, C.E., Mane,S.M., et al. (2008) RNA-seq: An assessment of technical reproducibility and comparison with gene expression arrays Genome Res.
Pickrell, J.K., et al. (2010) Understanding mechanisms underlying human gene expression variation with RNA sequencing. Nature.
The ENCODE Consortium. (2001) Standards, Guidelines and Best Practices for RNA-Seq.V1.0. (June 2011).
Wang, Z., Gerstein, M., Snyder, M. (2009) RNA-Seq: a revolutionary tool for transcriptomics. Nat. Rev. Genet.
DNA sample QC for NGS
Library QC for NGS
Assessment of library size
Determination of library quantity
Factors to consider in RNA-seq