Take a deep and exact look at any miRNA
Quantitative PCR is the tool of choice for analyzing known miRNA targets and offers several benefits over NGS. qPCR is fast, simple and inexpensive and provides extremely sensitive analyses from low amounts of input RNA. It also easily scales from screening and profiling to validation of NGS studies and verification of results from functional studies.
But miRNA qPCR presents special challenges. Their low abundance, short sequences, large variation in GC content and highly homologous families make designing very specific primers and probes difficult. We designed the miRCURY LNA miRNA PCR and Probe PCR systems to overcome these issues, so you can count on robust and highly sensitive miRNA profiles, even for your most demanding applications.
LNA-enhanced miRNA qPCR
How you can benefit
Workflow & products
Your choice of detection chemistry
We offer you the choice between SYBR-Green and hydrolysis probe-based miRNA detection, both providing highly sensitive and specific detection.
Fast and simple workflow
The miRCURY LNA miRNA PCR systems combine the speed and convenience of a universal reverse transcription (RT) reaction with the sensitivity and specificity of LNA-enhanced qPCR primers. The simple workflow delivers fast and accurate results for all sample types, including tissues, cells, exosomes and liquid biopsies.
What you need to get started
A universal reverse transcription kit (1) contains the chemistry for both SYBR Green and probe-based detection. Dedicated PCR mastermixes (3) for each system are combined with your choice of pre-designed or self-designed assays or panels (4). The optional RNA Spike-In Kit (2) lets you perform quality control of the RNA isolation, cDNA synthesis and PCR amplification steps, so you can identify any poor-quality samples early on, eliminate them from your study and save time and reagents.
|Detection system||SYBR Green||LNA-enhanced hydrolysis probe
|1||Reverse Transcription||miRCURY LNA RT Kit||miRCURY LNA RT Kit|
|2||Optional spike-in||RNA Spike-In Kit, For RT||RNA Spike-In Kit, For RT|
|3||Mastermix for qPCR||miRCURY LNA SYBR Green PCR Kits||miRCURY LNA Probe PCR Kits|
|4||Assay or panel||• miRCURY LNA miRNA PCR Assays
• miRCURY LNA miRNA Custom PCR Assays
• miRCURY LNA miRNA Focus PCR Panels
• miRCURY LNA miRNA Custom PCR Panels
• miRCURY LNA miRNA miRNome PCR Panels
• miRCURY LNA miRNA Probe PCR Assays
Which assay or panel is best for your study?
The miRCURY LNA miRNA PCR systems let you tailor the experimental setup to various types of studies with individual assays or fully flexible custom panels. You can use the same cDNA reaction and shift seamlessly between individual primers, predesigned miRNome and Focus panels or custom panels.
|Product Type||Advantage||Type of Study|
|Starter kit*||Few assays with a low number of samples||Getting started|
|Individual assays||Economical option for small-scale screening of a few miRNAs or
for quantification of a high number of samples
|Validation experiment with few miRNAs
|Custom panels||Pre-pipetted, custom-selected assays ensure high reproducibility ;||Focused screening or validation experiment|
|Focus panels||Pre-defined assay selections targeting certain research areas||Focused screening Focused screening|
|miRNome panels||Cover all important miRNAs||Genome-wide screening|