RNeasy Maxi Kit
For purification of up to 6 mg total RNA from cells, tissues, and yeast
The RNeasy Maxi Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns with a binding capacity of 6 mg RNA. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For smaller samples, the RNeasy Micro Kit (spin-column binding capacity of up to 45 µg RNA), the RNeasy Mini Kit (spin-column binding capacity of 100 µg RNA), and RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA) are also available.
Total RNA purified with the RNeasy Maxi Kit is of high quality and is suitable for many downstream applications (see figure "High-quality RNA from a variety of samples"). Total RNA is easily purified with the RNeasy Maxi Kit from large amounts of starting material including animal or human cells, animal or human tissues, and yeast cells (see table).
The RNeasy Maxi Kit delivers efficient purification of total RNA from large amounts of starting material. RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. Total RNA is easily purified from animal or human cells, animal or human tissues, and yeast. Protocols are included in the kit for cleanup of partially purified RNA, in vitro transcripts, and RNA from enzymatic reactions. RNeasy purified nucleic acids can be used in any molecular assay or other downstream application.
RNeasy technology simplifies total RNA isolation. Samples (up to 5 x 108 animal or human cells, 2.5 x 109 yeast cells, or 150 mg to 1 g tissue) are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy silica membrane (see figure "RNeasy Maxi spin column"). RNA (up to 6 mg) binds to the membrane, and all contaminants are efficiently washed away. Residual amounts of DNA can be removed using the convenient on-column DNase treatment. Pure, concentrated RNA is eluted in 800–2400 µl water (see figure "RNeasy Maxi procedure"). A variety of special application protocols is also available.
RNA purified with RNeasy technology has A260/280 ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5) and is ideal for use in all applications. Downstream applications include:
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