Penta·His HRP Conjugate Kit

For sensitive, direct detection of His-tagged proteins

High-sensitivity and high-speed detection of His-tagged proteins
No need for secondary antibody
Complete kit with optimized blocking reagent

The Penta·His HRP Conjugate Kit is for sensitive detection of recombinant proteins carrying His tags without the need for secondary antibodies. It consists of Anti·His antibodies conjugated to horseradish peroxidase (HRP). In detection procedures, the Anti·His antibody binds to a protein's His tag and the complex is localized using chemiluminescent or chromogenic substrates that react with HRP.

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Product Details
Specifications
Product Resources

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Cat No./ID: 34460

Penta·His HRP Conjugate Kit

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125 μl Penta·His HRP Conjugate, 5 g Blocking Reagent, 50 ml Blocking Reagent Buffer (10x concentrate)

The Penta·His HRP Conjugate Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Performance

Performance of Penta·His HRP Conjugate Kit
Property	Value
Detection:	N-terminal, C-terminal, and internal 6xHis tags
Sensitivity in dot blots:*	10 pg
Sensitivity in western blots:*	50 pg
Antibody Isotype:	Mouse IgG1
Cross-reactivity:	Negligible with crude E. coli, yeast, mammalian, or insect cell lysates
Form:	Stabilized stock solution
Working solution:	125–250 ml

Principle

The Penta·His HRP Conjugate Kit consists of the QIAexpress Anti·His Antibodies chemically coupled to the reporter enzyme horseradish peroxidase (HRP). It can be used for highly specific and sensitive, direct detection of any recombinant protein that has an accessible His tag. The Anti·His HRP Conjugate Kits eliminate the need for secondary antibodies, saving time in immunodetection procedures.

Procedure

The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.

Applications

The Penta·His HRP Conjugate Kit can be used with chemiluminescent or chromogenic detection substrates in:

Western, dot, and colony blotting procedures
ELISA and other immunoassay procedures

Specifications

Features	Specifications
Conjugated enzyme	HRP
Detection	Secondary antibody required
Epitope detected	HHHHH
Substrate for blot detection	4C1N
Substrates for assay procedure	OPD
Tag	His tag

Product Resources

FAQs (4)

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	What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize? FAQ ID -170	View
	Which Anti-His Antibody is the most sensitive for my protein of interest? FAQ ID -172	View
	Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies? FAQ ID -1073	View
	How can I avoid poor immunolocalization morphology with Anti-His Antibodies? FAQ ID -200	View

Kit Handbooks (1)

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	QIAexpress Detection and Assay Handbook - (EN) For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates	Show details

Quick-Start Protocols (1)

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	Immunodetection with Anti-His Antibodies or Anti-His HRP Conjugates (Chemiluminescent Method) (EN)	Show details

Safety Data Sheets (2)

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	MSDS Penta·His HRP Conjugate Kit
	CofA