miScript miRNome for biomarker discovery
For SYBR® Green-based real-time PCR analysis of miRNAs and discovery of those most important to the research area of interest
The miRNome is the most complete and accurate set of verified primers collected together in a single solution. Currently there are approximately thousands of miRNA that can be quantified using the QIAGEN miRNome. The content of miScript miRNA PCR Arrays is selected by updating our assay list to newly annotated miRNA found in miRBase, the database of miRNA, which ensures that each array is up-to-date and biologically relevant. These up-to-date miRNome miScript miRNA PCR Arrays are available for a variety of species including human, mouse, rat, dog and rhesus macaque.Use of the Human miRNome miScript miRNA PCR Array maximizes the likelihood of discovering miRNA sequences whose expression patterns correlate with the biological phenotypes under study. A set of controls included on each plate enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. The Human miRNome miScript miRNA PCR Array enables easy and reliable, SYBR Green-based real-time PCR analysis of the expression of up to 2400 miRNA sequences and discovery of those most important to the research area of interest.
miScript miRNome for biomarker discovery are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Discover biomarkers in serum or plasma samples
The range of miScript miRNA PCR Arrays is continually expanding to enable new discoveries about the roles of miRNAs in biological processes. Qiagen has taken biomarker discovery beyond the individual pathways to entire miRNA profiling with the miRNome. The content of miScript miRNA PCR Arrays is selected using our proprietary methodology, which ensures that each array is up-to-date and biologically relevant. One of the most exciting areas of current miRNA research involves the assessment of miRNAs present in serum or plasma samples. The discovery of relatively stable, extracellular miRNAs in serum and plasma has generated huge interest in the use of changes in these miRNA levels as potential non-invasive biomarkers for a variety of diseases. As a result, the Serum & Plasma miScript miRNA PCR Array has been developed. This array enables rapid profiling of the 84 most relevant miRNAs associated with serum and plasma (see figure "Analysis of expression of circulating miRNAs in serum or plasma").
Profile the human miRNome: start with a little, get a lot!
The Human miRNome miScript miRNA PCR Array profiles the expression up to 2500 of the most abundantly expressed and best characterized miRNA sequences in the human miRNA genome (miRNome) as annotated in miRBase Release 21 (www.mirbase.org). With less than 1 µg RNA, the entire human miRNome can be rapidly profiled (see figure "Whole miRNome profiling"). Although they are well characterized, each of these miRNA sequences can regulate one to several messenger RNA transcripts, and conversely one mRNA can be regulated by one to several miRNA sequences. Therefore, the complex role that any given known miRNA sequence plays has yet to be completely defined. Each assay in a miScript miRNA PCR Array has been bench verified to ensure sensitive and specific detection of mature miRNA by real-time PCR.
The miScript PCR System enables sensitive, specific miRNA quantification and profiling using SYBR Green real-time PCR. The miScript PCR System covers all the steps involved in conversion of RNA to cDNA and subsequent real-time PCR detection of miRNAs.
Mature miRNA profiling with the miScript PCR System uses the following components:
miRNA expression profiling with miScript miRNA PCR Arrays is straightforward and robust. First, prepare cDNA buffered with miScript HiSpec Buffer using the miScript II RT Kit. Second, add a premix of cDNA, miScript Universal Primer, QuantiTect SYBR Green PCR Master Mix, and RNase-free water to a miScript miRNA PCR Array. Third, run the reaction in a real-time PCR cycler. Finally, analyze the data using the miScript miRNA PCR Array Data Analysis Tool.
miScript miRNA PCR Array plate layout
miScript miRNA PCR Arrays are available in 96-well, 384-well and Rotor-Disc 100 formats (see figures "Pathway-Focused or miRNome miScript miRNA PCR Array layout for plate formats A, C, D, F, M", "Pathway-Focused miScript miRNA PCR Array layout for plate formats E, G", "miRNome miScript miRNA PCR Array and miScript miRNA HC PCR Array layout for plate formats E, G" and "miScript miRNA PCR Array layout for Rotor-Disc format R"). Each array contains several control assays. The purpose of these assays is described in the table below.
Controls in miScript miRNA PCR Arrays
C. elegans miR-39 miScript Primer
For more information on these controls, consult the miScript miRNA PCR Array Handbook.
miScript miRNA QC PCR Array plate layout
miScript miRNA QC PCR Arrays enable assessment of the quality of multiple cDNA samples using real-time PCR. miScript miRNA QC PCR Arrays are available in 96-well, 384-well, and Rotor-Disc 100 formats (see figures miScript miRNA QC PCR Array layout for plate formats A, C, D, F, miScript miRNA QC PCR Array layout for plate formats E and G, and "miScript miRNA QC PCR Array layout for Rotor-Disc format R"). Each miScript miRNA QC PCR Array includes replicates of C. elegans miR-39 miScript Primer Assay, 3 mature miRNA miScript Primer Assays (miR-16, miR-21, miR-191), 3 snoRNA miScript Primer Assays (SNORD61, SNORD95, SNORD96A), miRTC miScript Primer Assay and a positive PCR control. For more information, consult the miScript miRNA PCR Array Handbook. The 96-well plate and Rotor-Disc 100 formats allow quality control of up to 8 cDNA samples. The 384-well format allows quality control of up to 32 cDNA samples.
miScript miRNA PCR Array Data Analysis Tool
Free, easy-to-use data analysis software is available online for miScript miRNA PCR Arrays. Each miScript miRNA PCR Array comes with a dedicated data analysis tool. Once raw CT values are entered, the analysis tool automatically interprets the PCR array controls, performs relative quantification using the ∆∆CT method and presents the results in a variety of visual formats, allowing you to rapidly interpret your data.
miScript miRNA PCR Arrays are used as part of the miScript PCR System for:
fragment fix placeholder