The CALR RGQ PCR Kit is a ready-to-use kit for the detection of somatic mutations in exon 9 of the gene encoding CALR (cDNA annotation of specific region: c. 1091_1162) (1, 2). The kit uses real-time polymerase chain reaction (PCR) on the Rotor-Gene Q 5plex HRM. To identify Type 1 and Type 2 CALR mutations, an allele-specific amplification is achieved by ARMS (Allele Refractory Mutation System) technology. For the detection of minor variants of CALR mutations, primers and probes to amplify mutated target sequences are combined in the reaction mixes with an oligonucleotide that is 3’-blocked with the addition of a phosphate group which is specific for a wild-type targeted sequence, and prevents elongation of the PCR product (PCR clamping). To validate and control the qPCR reaction in the presence of human genomic DNA template, each CALR reaction mix includes primers and a probe to amplify an endogenous sequence of the ABL1 housekeeping gene. This control sequence is amplified through a multiplex PCR reaction in the presence of all CALR mutant and wild-type DNA. All necessary components are included in the run to provide accurate and sensitive detection of CALR mutations.