For automated purification of intracellular RNA (including miRNAs) from stabilized blood
Cat. No. / ID: 762635
The QIAsymphony PAXgene Blood RNA Kit provides fully automated purification of cellular RNA on the QIAsymphony SP. The new, improved kit provides higher-quality RNA, including miRNA. Used with PAXgene Blood RNA Tubes (available from BD and BD authorized distributors, cat. no. 762165) for sample collection and stabilization, the system provides a complete preanalytical solution, from sample collection and stabilization through to purification of high-quality RNA using proven magnetic particle technology.
The fully automated PAXgene Blood RNA procedure provides RNA yields comparable to the manual PAXgene Blood RNA procedure. Typical yields of RNA isolated from 2.5 ml healthy human whole blood (4.6 x 106 - 1.1 x 107 leukocytes/ml) are ≥3 μg for >95% of the samples processed with all PAXgene Blood RNA Kits. The automated PAXgene Blood RNA procedure provides high-quality RNA from different donor samples with enrichment of small RNA species (see figure " High-quality RNA from different donors"). Since yields are highly donor-dependent, individual yields may vary. The integrated DNase treatment ensures that genomic DNA contamination is minimized. Purified RNA is ready to use in downstream applications, such as quantitative, real-time RT-PCR.
The QIAsymphony PAXgene Blood RNA Kit provides fully automated purification of intracellular RNA on the QIAsymphony SP. Used with PAXgene Blood RNA Tubes (available from BD and BD authorized distributors, cat. no. 762165) for sample collection and stabilization, the system provides a complete preanalytical solution from sample collection and stabilization through to purification of high-quality RNA using proven magnetic-particle technology. Prefilled cartridges reduce manual handling to a minimum, and bar code labeling enables full reagent tracking.
RNA purified using the QIAsymphony PAXgene Blood RNA Kit and the QIAsymphony SP is highly pure and ready for use in a wide range of sensitive downstream applications, including:
Blood was collected from 12 donors into PAXgene Blood RNA Tubes. RNA was purified using the QIAsymphony PAXgene Blood RNA Kit on the QIAsymphony SP. RNA was analyzed with an Agilent 2100 Bioanalyzer. Clear enrichment of small RNA species is shown in the gel-like image by the corresponding peaks in the donor RNA next to the marker band.
| Features | Specifications |
| Applications | Quantitative, real-time RT-PCR, expression array and chip analysis, next-generation sequencing, cDNA synthesis, RNase and S1 nuclease protection, northern, dot, and slot blot analysis, primer extension |
| Time per run | 72 samples in 6 h 15 min |
| Processing | Automated on the QIAsymphony |
| Format | Magnetic beads |
| Sample amount | 2.5 ml |
| Yield | 8–16 µg |
| Main sample type | Whole blood |
| Elution volume | 80 µl or 120 µl or 200 µl |
| Technology | Magnetic particles technology |
