For automated purification of total RNA, or total RNA with miRNA, from tissues or cells
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Cat. No. / ID: 931636
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
The QIAsymphony RNA Kit enables automated purification of total RNA, or total RNA including miRNA and other small RNAs, from 1–96 samples on the QIAsymphony SP. Total RNA can be purified from animal cells, tissues, or FFPE tissues, using very small amounts of starting material (≥100 cells) up to 1 x 107 cells or 50 mg of tissue. Optimized protocols provide high yields of pure total RNA, or total RNA including miRNA, and flexible elution volumes allow RNA concentration to be easily optimized for each downstream application. Prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes. The cartridges are automatically opened by the QIAsymphony SP helping to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control. The purification procedure comprises 4 steps: lyse, bind, wash, and elute. A number of protocols is available for the QIAsymphony SP for purifying RNA, depending on the starting material and the required RNA product (see table).
| Protocol | Running with | Sample material (Pretreated sample lysates) |
Processed volume (μl) |
| RNA CT 400 | QIAsymphony RNA Kit (2 x 96)‡ | ≤3 x 106 cultured cells ≤20 mg easy-to-lyse tissue |
400 μl |
| RNA CT 800 | QIAsymphony RNA Kit (2 x 48)‡ | 3 x 106–1 x 107 cultured cells ≤50 mg easy-to-lyse tissue |
800 μl |
| RNA FT 400* | QIAsymphony RNA Kit (2 x 96)‡ | ≤20 mg fibrous tissue | 400 μl |
| miRNA CT 400* | QIAsymphony RNA Kit (2 x 96)‡ | ≤3 x 106 cultured cells ≤20 mg tissue |
400 μl |
| RNA FFPE 130† | QIAsymphony RNA Kit (2 x 72)‡ | 1–2 x FFPE sections up to 10 μm 1 x FFPE section 20 μm |
130 μl |
The QIAsymphony RNA Kit, and fully automated purification of total RNA on the QIAsymphony SP, provides high-quality RNA suitable for direct use in any downstream application.
RNA was purified from Jurkat cells on the QIAsymphony SP using the QIAsymphony RNA Kit. RNA yields from 2 instrument runs using 2 different reagent cartridge lots were compared with manual purification using the RNeasy Mini Kit. Each bar represents the average of 4 samples.
| Features | Specifications |
| Applications | RT-PCR; Real-Time PCR; Expression Profiling |
| Sample amount | 400 µl lysate from <20 mg tissue or 3 x 10e6 cells or 800 µl lysate from <50 mg tissue or 3 x 10e6 cells |
| Elution volume | 50-200 µl |
