Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes. The cartridges are automatically opened by the QIAsymphony SP helping to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control. The purification procedure comprises 4 steps: lyse, bind, wash, and elute. A number of protocols is available for the QIAsymphony SP for purifying RNA, depending on the starting material and the required RNA product (see table).
Protocols for purification of RNA with the QIAsymphony SP instrument
Protocol |
Running with |
Sample material (Pretreated sample lysates) |
Processed volume (μl) |
RNA CT 400 |
QIAsymphony RNA Kit (2 x 96)‡ |
≤3 x 106 cultured cells ≤20 mg easy-to-lyse tissue |
400 μl |
RNA CT 800 |
QIAsymphony RNA Kit (2 x 48)‡ |
3 x 106–1 x 107 cultured cells ≤50 mg easy-to-lyse tissue |
800 μl |
RNA FT 400* |
QIAsymphony RNA Kit (2 x 96)‡ |
≤20 mg fibrous tissue |
400 μl |
miRNA CT 400* |
QIAsymphony RNA Kit (2 x 96)‡ |
≤3 x 106 cultured cells ≤20 mg tissue |
400 μl |
RNA FFPE 130† |
QIAsymphony RNA Kit (2 x 72)‡ |
1–2 x FFPE sections up to 10 μm 1 x FFPE section 20 μm |
130 μl |