PCR overview | Reproducibility | End-point, qPCR, dPCR

PCR Technologies

The reproducibility issue
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a growing concern affecting many scientists

Publication of reliable and reproducible studies sets the foundation for future scientific endeavors to build upon. However, many scientists cannot reproduce their peers’ results and sometimes even their own results. This irreproducibility crisis wastes valuable time and resources and can damage scientific credibility. Fortunately, you can help tackle the issue and promote high-quality science by using standardized PCR methods.

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Producing meaningful results
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Minimize PCR errors and variabilities

Avoid handling variations
- Multi-step procedures increase variables
- Undetected human errors can have a big downstream impact
Remove biological variables
- Contaminating biomolecules alter results
- Sample to sample variations lead to misinterpretation
- Appropriate controls alert you to performance discrepancies
Select appropriate chemistry
- Meaningful results require robust and reliable components
- Choose assay formats with foolproof procedures

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End-point PCR

Today’s research applications require more from PCR: increased throughput, higher sensitivity and reliable data analysis. Although it is one of the most commonly used lab methods, many researchers still worry about the complexity of designing PCR experiments. QIAGEN’s end-point PCR solutions remove your worries because we’ve built them to get every PCR job done correctly – the first time.

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Real-time qPCR

Our real-time PCR and RT-PCR portfolio is one of the most advanced. A unique combination of in-process controls removes variables and errors and streamlines your work. Combine this with an unparalleled catalog of predesigned PCR assays and panels, plus the ability to customize your own using our advanced design algorithms. The result? Precise and reproducible quantification and profiling of any miRNA, mRNA or lncRNA targets, with no hassle.

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Which PCR instrument fits your needs?

Discover QIAGEN’s new high-performance standard and real-time thermal cyclers

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Digital PCR

Prepare your critical research applications for a digital future. Digital PCR is a highly precise approach to absolute nucleic acid quantification without standard curves. It is ideal for identifying faint genetic signals against a strong background, as needed in sensitive applications like rare mutation detection. Our fully integrated nanoplate-based dPCR system combines superior partitioning with the ease of use of qPCR. It offers several advantages over qPCR, such as improved precision and accuracy, increased resistance to inhibitors and high reproducibility.

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Good planning is key to research success

Looking for a simple way to tailor your PCR assays to your research questions? The GeneGlobe Design & Analysis Hub is a powerful tool that streamlines the entire research planning process. With GeneGlobe, you can quickly and easily explore targets and pathways in their scientific context, find and customize the right products to study those targets, analyze the data and plan follow-up studies.

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Back to the PCR basics

Are you new to PCR or do you need to refresh your memory of the technology? Explore the PCR bench guide, our comprehensive PCR knowledge base. Find guidelines and suggestions for maximizing your PCR results along with protocols, hints and tips.

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Maximizing PCR success