How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
FAQ ID -2356

The master mix supplied with the QuantiFast Probe RT-PCR Plus Kit contains no ROX dye. In order to adjust the reaction for instruments from Applied Biosystems (models 7000, 7300, 7700, 7900HT, StepOne™, and StepOnePlus™ Real-Time PCR Systems), it is necessary to add High-ROX Dye Solution to the master mix. For Applied Biosystems 7500 and instruments from Stratagene, ROX dye is required at a lower concentration. This requires the user to add the supplied ROX Dye Solution to the master mix during reaction setup (see the kit handbook for details).