How do Unique Molecular Indexes (UMIs) improve quantification?
FAQ ID - 3669

During the QIAseq miRNA Library Kit library construction process, each individual miRNA molecule is tagged with a UMI. Following sequencing, raw reads are mapped to individual miRNA molecules instead of just individual miRNAs. This allows for true quantification of the miRNAs by eliminating any library amplification and sequencing bias.