What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?
FAQ ID -2010

The EpiTect MethyLight PCR Kit chemistry is especially developed for the requirements of bisulfite converted DNA. As a result of methylation and the conversion of unmethylated cytosines through bisulfite treatment, probe hybridization behavior differs from that of untreated DNA, and requires special buffer conditions. The balanced cations as well as Factor MP in the MethyLight PCR chemistry are contributing to the special hybridization behavior required for bisulfite converted DNA.