Log in To see your account pricing.
Need bulk, customized or optimized products for commercial purposes? We also offer support with logistics, compliance and more. Reach out to cooperate with QIAGEN Strategic Partnerships & OEM
dNTP Set, PCR Grade, 4 x 100 µL
Cat no. / ID. 201912
100 mM each dATP, dCTP, dGTP, dTTP for 1000 x 50 µL PCR reactions
Need bulk, customized or optimized products for commercial purposes? We also offer support with logistics, compliance and more. Reach out to cooperate with QIAGEN Strategic Partnerships & OEM
Features
- Individual dATP, dCTP, dGTP and dTTP supplied separately
- Highly pure nucleotides for reproducible amplification
- Allows custom mixing and concentration adjustment
- Supplied in 100 mM aqueous solutions
- Suitable for standard, sensitive and specialized PCR applications
- Compatible with PCR and RT-PCR enzymes
Product Details
dNTP Set consists of individual, highly pure deoxynucleotide triphosphates at 100 mM concentration in aqueous solution. Supply of each dNTP at high concentration and purity enables customized reaction setup for standard, sensitive and specialized PCR applications. High-purity dNTPs are essential for sensitive PCR where contaminating impurities may reduce sensitivity, amplification efficiency and product yield.
A set of individual dNTPs allows adjustments to the concentrations of specific nucleotides. This is necessary for certain labeling techniques, and desirable for flexibility to troubleshoot and alter reaction components.
A combined dNTP ready-to-use product is available. dNTP Mix contains dATP, dCTP, dGTP and dTTP in water (pH 7.5), each at a concentration of 10 mM. This product is suitable for use in all standard PCR techniques and sensitive PCR techniques.
| Feature | dNTP Set | dNTP Mix |
|---|---|---|
| Best for | Custom nucleotide formulations, assay optimization, and applications requiring full control over nucleotide composition | Routine workflows and standardized assays requiring high reproducibility |
| Why choose it | Maximum flexibility for protocol optimization | Ready-to-use convenience with consistent, reproducible results |
| Flexibility | Yes – customize nucleotide composition and ratios | No – fixed, optimized 1:1:1:1 nucleotide ratio |
| Ease of use | Manual preparation required | Ready to use with minimal hands-on preparation |
| Format | 100 mM each dATP, dCTP, dGTP, and dTTP | Premix containing 10 mM each dATP, dCTP, dGTP, and dTTP |
| Available volumes | 4 × 100 µL 4 × 250 µL |
200 µL 800 µL |
| Catalog numbers | 201912 201913 |
201900 201901 |
Performance
The performance of each lot of dNTP Set is tested in a specific polymerase chain reaction requiring all four nucleotides.
Principle
dNTP Set is ideal for customized labeling and specialized PCR applications by allowing adjustment of individual deoxynucleotide ratios and concentrations.
Procedure
Prepare standard PCR assays with a typical 200 µM concentration of each dNTP. Manually formulate a specific dNTP mix to free up space for a labeled nucleotide in applications like custom labeling in standard PCR and RT-PCR using, for example, HotStarTaq or QIAGEN OneStep RT-PCR Kit, or in enzymatic labeling procedures with Klenow Fragment (3'→5' exo-). Minimize PCR carryover contamination by substituting dTTP with dUTP in the primary amplification and treating with thermolabile Uracil DNA Glycosylase (UDG) before the new PCR reaction starts.
Instructions for the use of dNTP Set can be found in the appropriate QIAGEN PCR kit handbook or in product protocol documents.
Applications
Each dNTP of dNTP Set can be diluted and bases combined as required, providing high-purity nucleotides for maximum flexibility in standard and specialized PCR workflows, DNA labeling protocols and assay optimization.
- Standard PCR
- High-sensitivity PCR
- Long-range and high-fidelity PCR
- Multiplex PCR
- Single-cell PCR
- Genotyping
- Methylation-specific PCR (MSP)
- Two-step RT-PCR
- DNA labeling with modified dNTPs
- Minimizing PCR cross-contamination
- DNA sequencing