Pyrosequencing Applications

Quantitative data for new horizons in genetic research

Pyrosequencing is a unique detection technology based on the principle of sequencing-by-synthesis, which provides quantitative real-time data without the need for gels, probes, or labels. In addition to characterizing single nucleotide polymorphisms (SNPs), insertion-deletions (indels), and unknown sequence variants, it can sensitively quantify allele frequencies and DNA methylation levels at both CpG and non-CpG (CpN) sites. Integrating detection and quantification of genetic variation into one powerful system, Pyrosequencing with the PyroMark platform outperforms other sequence-based solutions in the analysis of targeted short DNA sequences. For a range of applications, this means reduced cost and time investment.