This webinar provides general considerations for performing a gene expression analysis on the QIAcuity digital PCR system, including sample input, dilutions and proper controls. We will present how the QuantiNova LNA PCR Assays provide highly sensitive and accurate locked nucleic acid (LNA)-enhanced digital PCR quantification for mRNA and lncRNA targets, detecting even the smallest expression changes at the lowest concentrations.

We will further demonstrate how to analyze gene expression data with accuracy and reliability using the QIAcuity Software Suite and generate publication-quality fold change and fold regulation results.

About the speaker
Dr. Ronny Kellner, Senior Scientist, Digital PCR Assay Development
Ronny Kellner studied biology at the Eberhard Karls University in Tübingen. He completed his Ph.D. in molecular plant-pathogen interaction at the MPI for Terrestrial Microbiology in Marburg and the Ruhr-University of Bochum. After working on several international projects in Germany and the UK focusing on microbiology, molecular biology and NGS data analysis, he joined QIAGEN in August 2020. Since then, he has been involved in developing the digital PCR assay portfolio, including gene expression assays.
Date of recording:Thursday, October 22, 2020
Duration:1 hour
Digital PCR
Molecular Biology Research