OEM custom enzymes
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OEM by QIAGEN

Custom Enzymes

Deep dive into OEM custom enzymes and bulk manufacturing capabilities

Assay developers are challenged with consolidating the supply chain, while ensuring top-quality components for a consistent end-product. OEM by QIAGEN can help simplify this process by providing a wide range of enzymes in bulk. Custom enzyme manufacturing can be the key to successfully bringing a commercial assay to market. A dedicated project manager will work with you from the start. We can tailor enzymes to your specifications, including sampling and testing. Check out our broad enzyme portfolio. Manufacturing quantities can grow with your business. Allow us to be your trusted partner for OEM enzymes.

 

Enzyme Portfolio Overview

Product name VeraSeq 2.0 High Fidelity DNA
Polymerase
VeraSeq Ultra DNA Polymerase Phoenix Hot-Start Taq DNA
Polymerase
Taq DSC 2.0 DNA Polymerase
Description For maximized DNA
amplification speed, accuracy,
and length
To read and use Uracil residue
with maximized speed and
accuracy
For enhanced specificity,
sensitivity, and yield
A nucleic acid based hot start
polymerase with instant
activation
Features • 1kB in 15 secs.
• Fidelity 50x higher than Taq
• Blunt end
• 1.5-3.0mM Mg2+
concentrations
• 1kB in 15 secs.
• Fidelity 25x higher than Taq
• Blunt end
• 1.5-3.0mM Mg2+
concentrations 
• Reduced nonspecific
amplification and primer-dimer
formation
• Room temperature reaction
set up concentrations
• Thermostable, processive
DNA polymerase
• No 3' to 5' proofreading
• Hot start
Applications Routine HiFi amplification,
NGS library amplification and
synthetic biology
Bisulfite-Seq, contamination
prevention, long-range
Routine PCR, qPCR,
multiplexing, RT-PCR
Routine PCR, qPCR,
multiplexing, RT-PCR
Exonuclease
activity
3'➞5' 3'➞5'  5'➞3' 5'➞3' 
Hot-start Antibody based Nucleic acid based
Thermostable Ultra-thermostable Ultra-thermostable
Proof reading
High fidelity
Long range
Lyo-ready,
glycerol-free
Product name Taq-B DNA Polymerase TaqIT DNA Polymerase Phi29 DNA Polymerase BstX DNA Polymerase
Description Wild-type Taq DNA
polymerase
Exonuclease deficient
derivative of Taq DNA
polymerase 
Highly processive DNA
polymerase, for accuracy and
speed 
Thermostable DNA
polymerase for better
isothermal amplification
Features • 5' to 3' exonuclease activity
• 5' to 3' nick translation
moiety No 3' to 5'
proofreading 
• Slightly greater fidelity,
inhibition resistance and
thermostability than full length
Taq 
• Strong strand displacement
activity and processivity up to
70,000 base insertions per
binding event 
• Strong strand displacement
• High salt and detergent
tolerance 
Applications Industry standard for routine
PCR
Longer PCR amplification,
genotyping, allows bypassing
of DNA extraction and
purification steps 
Whole Genome Amplification
(WGA), Rolling Circle
Amplification (RCA), Multiple
Displacement Amplification
(MDA)
LAMP, strand-displacement
Synthesis ideal for isothermal
amplification 
Exonuclease
activity
5'➞3' 3'➞5'
Hot-start      
Thermostable High thermostability  
Proof reading    
High fidelity
 
Long range  
Lyo-ready,
glycerol-free
Enzyme category Binding Protein Other Other
Product name T4 gene 32 Protein Uracil DNA Glycosylase 10x Uracil Cleavage System
Description Wild-type For ssDNA stabilization and
increased processivity
Uracil DNA glycosylase for removing
Uracil from DNA 
Uracil DNA glycosylase (UDG) and
Endonuclease VIII
Features • A single-stranded DNA binding protein • Catalyzes the hydrolysis of the
N-glycosylic bond between the uracil
and sugar 
• Removal of uracil, then cleavage of
DNA backbone
Applications DNA sequencing in secondary structure
rich regions and PCR amplification
Creating a basic sites, containing single
or double-stranded DNA 
Cloning, removing contaminating PCR
products
Exonuclease
activity

 
Lyo-ready,
glycerol-free

Product name Poly(A) Polymerase T7 RNA Polymerase
Description Catalyzes addition of AMP from ATP to the 3" hydroxyl of RNA Synthesizes RNA in 5'➞3' direction of DNA template
Features • Template independent catalysis of the addition of AMP from
ATP to the 3' hydroxyl of RNA
• DNA-dependent RNA polymerase with high specificity to T7
promoter 
Applications Addition of poly(A) tail to RNA, 3'-end RNA labeling RNA synthesis from DNA template, RNA probe preparation,
applications in mRNA therapeutics 
Reverse Transcriptase
Product name Omniscript RT Sensiscript EnzScript (MMLV
Reverse Transcriptase
RNase, H-)
RNase inhibitor RNase H
Description For viral RNA
preparation with
carrier use
For highly sensitive
applications with very
small amounts of RNA
(<50 ng) 
RNA-dependent DNA
polymerase
Porcine-derived
non-competitive
RNase A, B, C inhibitor 
Cleaves RNA strand
of DNA-RNA hybrid
Features • Efficient and
sensitive RT with high
cDNA yields from
small amounts of RNA 
• Efficient and
sensitive RT
• For use with less
than 50 ng of RNA 
• No RNase H activity
• cDNA transcripts >
5kb
• Reaction
• Does not inhibit
RNase H activity
• Degrades the RNA
strand of RNA-DNA
hybrid
• Does not degrade
single or double
stranded RNA
Applications cDNA synthesis,
one-step RT-PCR
Single-cell RT-PCR,
small biopsies analysis,
cDNA synthesis,
one-step RT-PCR 
cDNA synthesis,
one-step RT-PCR,
RNAseq
cDNA synthesis,
one-step RT-PCR 
Removes mRNA during
second strand cDNA
synthesis
Long range

Lyo-ready,
glycerol-free


DNA modifying enzymes
Product name DNA Polymerase I Klenow Fragment Klenow (3'-5'exo-)
Fragment
Mako DNA
Polymerase
T4 DNA
Polymerase
T7 DNA
Polymerase
Description Mesophilic DNA
polymerase 
Truncated product
of E. coli DNA
polymerase I 
A derivative of
E. coli DNA
polymerase I
Exonuclease-
deficient
polymerase 
Extension of a
primed DNA
template in 5'➞3'
direction
Highly processive
DNA polymerase
Features • 5'➞3' DNA
synthesis
• 5'➞3'
polymerase 
• 5'➞3'
polymerase
• No strand
displacement
activity
• Polishing 5' and
3' of ends
• No strand
displacement
• Exceptionally
high synthesis and
replication fidelity
rate
Applications DNA labeling by
nick translation,
cDNA synthesis
DNA blunting by
fill-in 5’ overhang,
second strand
cDNA synthesis,
sequencing and
site-specific
mutagenesis 
A-tailing for
NextGen
sequencing, strand
displacement
amplification,
DNA labeling
Sequencing Double-stranded
DNA labeling 
Highly processive,
site-specific
mutagenesis,
second strand
cDNA synthesis
Exonuclease
activity
Both 3'➞5' and
5'➞3'
exonuclease
activity
3'➞5'
exonuclease
activity, no 5'➞3'
exonuclease
activity
Deficient in
proofreading
(3'➞5') and
nick-translation
(5'➞3') nuclease
activities
No 3'➞5' or
5'➞3'
exonuclease
activity
Powerful 3'➞5'
exonuclease
activity, no 5'➞3'
exonuclease
activity
3'➞5'
exonuclease
activity
Lyo-ready,
glycerol-free



 
Product name T4 Polynucleotide
Kinase
Terminal
Deoxynucleotidyl
Transferase
Thermostable
Pyrophosphatase
End Repair Mix E. coli fpg Endonuclease VIII
Description 5' phosphorylation
of DNA or RNA,
removes 3'
phosphoryl groups
A template-
independent DNA
polymerase 
Catalyzes the
hydrolysis of
inorganic
pyrophosphate to
produce
orthophosphate
Combination of T4
DNA Polymerase
and T4 PNK 
Participates in the
base-excision
pathway of DNA
repair enzymes
DNA repair
protein, removes
modified
pyrimidines
Features • Exhibits
3'-phosphatase
and 2', 3' cyclic
phosphodiesterase
activities 
• Catalyzes
deoxynucleotides
addition to the 3'
hydroxyl terminus
of single or double
stranded DNA 
• Eliminates
pyrophosphate
during
amplification,
thus minimizing
inhibition
• Functional under
PCR conditions
• Converts DNA
with 5'- and/or
3'-protruding
ends to 5'-
phosphorylated,
blunt-ended DNA.
(in high/low
concentrations) 
• Acts both as a
N-glycosylase and
an AP-lyase
• Possesses both
DNA glycosylase
and apurinic/
apyrimidinic (AP)
lyase activities
Applications Phosphorylation of
5' ends of DNA
prior to ligation
Homopolymeric
tailing to the 3'-
OH, TUNEL assay,
5’-RACE, labeling
of 3’-end 
In-vitro synthesis Generate
blunt-ended DNA
for cloning or
NGS library
preparation 
Excises damaged
purines from
duplex DNA,
cleaves AP sites
leaving 3' and 5'
phosphates
Excises damaged
pyrimidines from
duplex DNA,
cleaves AP sites
leaving 3' and 5'
phosphates
Thermostable
Lyo-ready,
glycerol-free

NGS applications
NGS library preparation
Product name 5X WGS Fragmentation Mix 5X ER/A-Tailing Enzyme Mix
Description A single tube solution for library construction on Illumina
platforms
A NGS library preparation module
Features • Fragmentation, end-repair and dA-tailing in a single
reaction step
• Ligation optional in the same tube
• Profiles similar to mechanical shearing Modifiable
fragment size
• Input DNA from 1ng to 1ug, with various GC contents
• End-repair and dA-tailing in a single reaction step
• Input DNA from 250 pg to 1 ug
• Compatible with EDTA
• Total time under 3 hours/1 hour hands-on time
• Even coverage across genomic regions
• PCR free library prep 
NGS Illumina library
construction
From intact DNA From fragmented DNA
Lyo-ready, glycerol-free Feasible
NGS applications
Additional modifications
Product name Restriction enzymes Exonucleases
Description Example Products: BamHI, BglII, DpnI, EagI, EcoRI, EcoRV, HaeIII,
HindIII, NcoI, NotI, PstI, PvuII, SalI, SphI, XbaI, XhoI
Example Products in the exonuclease family are: Exonuclease I, III
and Lambda exonuclease
Features • Cleaves double stranded DNA within target recognition sites • Exonuclease I cleaves single-stranded DNA in the 3'➞5'
direction
• Exonuclease III acts in 3’-5’ direction on double stranded DNA
substrate
• Lambda Exonuclease is a highly processive 5'➞3' double
stranded exonuclease
Applications Endonuclease application: cloning and restriction site mapping Removal of chromosomal DNA to clean up plasmid preps,
generation of ssDNA from linear dsDNA, removal of
oligonucleotide primers post PCR
Product name WGS Ligase T3 DNA Ligase T7 DNA Ligase E. coli DNA Ligase T4 DNA Ligase
Description Optimized for ligation
following WGS
Fragmentation
ATP-dependent dsDNA
ligase. Catalyzes the
formation of a
phosphodiester bond
between a 5'
phosphate and a 3'
hydroxyl termini in
duplex DNA 
Catalyzes the
formation of a
phosphodiester bond
between a 5'
phosphate and a 3'
hydroxyl termini in
duplex DNA
An NAD+-dependent
enzyme 
Catalyzes the
formation of
phosphodiester bond
between the terminal
5’ phosphate and 3’
hydroxyl groups of
duplex DNA or RNA
Features • Joins blunt end and
cohesive ends and
• Repairs single
stranded nicks in
duplex RNA, RNA, or
DNA-RNA hybrid 
• Joins blunt end and
cohesive ends and
repairs single stranded
nicks in duplex DNA 
• Joins blunt end and
cohesive ends and
repairs single stranded
nicks in duplex DNA
• Ligates DNA at nicks
and cohesive termini
• Joins blunt end and
cohesive ends and
• Seals nicks in duplex
DNA, RNA or
DNA-RNA hybrid
Applications NGS library
construction, cloning 
dsDNA ligase that
works in high ionic
strength up to 1.0 M
NaCl 
High cohesive end
ligation efficiency
cDNA cloning by
replacement synthesis 
Restriction cloning, TA
cloning, adapter
ligation
Lyo-ready,
glycerol-free
lyophilized
Feasible
Product name Taq DNA Ligase T4 RNA Ligase 1 T4 RNA Ligase 2 T4 RNA Ligase 2 Truncated
Description Thermostable DNA ligase Ligates single-stranded RNA
and single stranded DNA
molecules
Ligates nicks on double
stranded DNA
Ligates pre-adenylated 5'
phosphate DNA or RNA and
3' hydroxyl of RNA
Features • Seals nicks and
discriminates against
mismatch ligation 
• Catalyzes ATP-dependent
ligation of single-stranded
nucleic acids 
• Ligates from 3' OH of RNA
to 5' phosphate of DNA in
double stranded structures
• Linker ligations with
pre-adenylated 5' DNA to 3'
hydroxyl RNA 
Applications Ligase Chain Reaction and
Ligase Detection Reaction,
high temperature ligation
Single-stranded nucleic acid
(RNA or DNA) ligation,
labeling of 3'-termini of RNA 
Nick ligation in dsRNA, Ligate
the 3' OH of RNA to the 5'
phosphate of DNA in a
double stranded format
NGS RNA library construction
(miRNA-Seq or directional
mRNA-Seq) 
Thermostable  
High fidelity
   
Product name QuantiNova Multiplex PCR Kit QuantiNova Probe PCR Kit QuantiTect Probe PCR Kit QuantiTect Multiplex PCR Kit
Description Ultrafast multiplex, real-time
PCR and two-step qRT-PCR
using sequence-specific
probes
Highly sensitive, specific, and
ultrafast PCR mastermix for
probe-based real-time PCR
Chemically inactivated
hot-start PCR mastermix for
real-time PCR using
probe-based detection
Probe-based detection
Features • Hot-start
• Sensitive detection of up to
five targets in one tube
• Visual pipetting control
• Room temperature reaction
set up
• Hot-start
• Detection of rare targets
down to one copy
• Visual pipetting control
• Room temperature reaction
set up 
• Hot-start
• Very high PCR specificity
• dUTP for pretreatment with
uracil-N-glycosylase (UNG)
• Hot-start
• Very high PCR specificity
• Multiplex capability up to
five targets
• dUTP for pretreatment with
uracil-N-glycosylase (UNG) 
Applications Multiplex gene expression
analysis of cDNA or gDNA
targets on any real-time cycler
Probe-based gene expression
analysis of cDNA targets
and quantitative gDNA
analysis on any real-time
cycler 
Real-time PCR of cDNA or
gDNA targets with duplex
capability
Multiplex real-time PCR 
Hot-start Antibody mediated Antibody mediated Chemical modification Chemical modification
Real-time PCR
Multiplex PCR
Product name UltraRun LongRange PCR Kit AllTaq Master Mix Kit HiFi PCR Master Mix
Description Accurate ultra-long-range PCR Sensitive and specific Hot-start PCR  High fidelity PCR amplification of NGS
libraries
Features • Up to 40 kb
• Hot-start
• Low error rates, ensured by high-fidelity
enzyme
• Room temperature stability
• One protocol for all targets
• GC-rich or up to 9 kb
• Duplex PCR
• Visual pipetting control
• Gel tracking dyes 
• Highly efficient and low bias PCR
master mix
• Uniform coverage across challenging
AT- and GC-rich regions
• Input DNA ≥250 pg
Applications Cloning, sequencing, standard PCR PCR, genotyping and genetic analysis
with multiple primer pairs or wide range
of amplicon sizes 
DNA or RNA seq library amplification,
including single cell RNAseq
Hot-start Antibody mediated Antibody mediated Antibody mediated
End-point PCR
Multiplex PCR
Long range

Contains HiFi in
enzyme blend

UltraClean® Production
Product name UCP Probe PCR Kit UCP Multiplex PCR Kit UCP HiFidelity PCR Kit
Description Probe-based, UCP PCR UCP Nucleic acid depleted PCR  High fidelity, hot-start UCP PCR
Features • Nucleic acid-depleted reagents, no
background from residual DNA.
• Inhibitor robustness
• Visual pipetting control
• Room-temperature reaction setup
• No background from residual DNA
• Inhibitor robustness
• Wide GC range
• Visual pipetting control
• Gel tracking dyes
• Room temperature reaction setup 
• Blunt end products
• Up to 10 kb
• Inhibitor robustness for direct PCR from
blood samples
• Fidelity 70 times higher than Taq
• Room temperature reaction setup
Applications Microbial testing, microbiome/
metagenome workflows, NGS library
preparation
Microbial testing, quality control,
genotyping and genetic testing,
microbiome/metagenome analysis and
sequencing, 16S/18S amplifications 
Microbial testing, quality control,
genotyping and genetic testing,
microbiome/metagenome analysis and
sequencing, 16S/18S amplifications
Hot-start Antibody mediated Antibody mediated Antibody mediated
Real-time PCR    
End-point PCR
Multiplex PCR
Ultra Clean
Production (UCP)


Room temperature
reaction setup
Inhibitor
robustness
Contains HiFi in
enzyme blend
Product name QuantiNova Pathoge + IC Kit QIAGEN One Step Ahead
RT-PCR kit
QuantiNova Probe RT-PCR Kit QuantiNova Multiplex RT-PCR
Kit
Description Real-time multiplex RT-PCR kit
for probe-based pathogen
detection
For highly sensitive and
specific RT-PCR using any
RNA template
For one-step qRT-PCR using
sequence-specific probes
for gene expression analysis
Quantification of up to five
RNA targets in a single tube
by multiplex real-time RT-PCR
Features • Ultrafast, simultaneous
detection of pathogen DNA
and RNA
• Room temperature reaction
setup
• Visual pipetting control
• One-step RT-PCR
• Room-temperature setup
• Duplex capability 
• Internal control for positive
in-process verification
• Duplex capability
• Room temperature reaction
setup
• Visual pipetting control
• Amplification from a single
cell or up to 800 ng template
• Internal control RNA for
verification
• Room temperature reaction
setup
• Visual pipetting control 
Applications 4-plex RT-PCR for detection of
both pathogen targets and
internal control
RT-PCR applications for virus
detection, gene expression
analysis 
Gene expression analysis of
RNA targets on any real-time
cycler
 
Real-time PCR
End-point PCR      
Multiplex PCR      
Room temperature
reaction setup
Hot-start Antibody mediated hotstart
Taq, inactivated RT
Antibody mediated hotstart
Taq, inactivated RT
Antibody mediated hotstart
Taq, inactivated RT
Antibody mediated hotstart
Taq, inactivated RT
Product name QuantiTect Probe RT-PCR Kit QuantiTect Multiplex RT-PCR Kit ZipScript™ One-Step RT-qPCR Kit
Description For one-step RT-qPCR using
sequence-specific probes for gene
expression analysis
For RNA template using the SYBR®
Green detection 
A highly sensitive and reproducible
RT-qPCR solution optimized for real-time
PCR
Features • Sensitive detection of low-copy targets
• Use of any sequence-specific probe
on any real-time cycler
• dUTP for pretreatment with
uracil-N-glycosylase (UNG)
• Omniscript/ Sensiscript mix for cDNA
synthesis
• dUTP for pretreatment with
uracil-N-glycosylase (UNG)
• Up to five targets in one reaction 
• The 25X enzyme mix is accompanied
by a 2X reaction buffer
• Capable of multiplexing up to five
targets
• Can be obtained lyophilized
Applications Real-time quantification of RNA targets One-step real-time PCR (1-step RT-qPCR)  Gene expression for RNA template,
1-step RT-qPCR
Real-time PCR
Multiplex PCR
Hot-start Chemical modification Chemical modification Antibody mediated
Formulation capabilities Consulting service
• Adjusted concentration
• Lyophilized or in the buffer of your choice
• Bulk or aliquots in tubes
• Customized mixing
• Tailored labeling and packaging, flexible volumetric ordering
• Implement tailored modifications and design
• Contract protein manufacturing
• Stability studies
• Custom mixes, buffer, and diluent support
• R&D prototyping of proteins and formulations for ease of evaluation
Interested in OEM? Contact us now
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