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Penta·His Alexa Fluor 488 Conjugate
Cat. No. / ID: 35310
125 μl Penta·His Alexa Fluor 488 Conjugate, 200 μg/ml
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Emission at
488 nm
647 nm
Penta·His Alexa Fluor Conjugates are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Highly specific and sensitive detection of His-tagged proteins
- Direct detection without the need for a secondary antibody
- Broad instrument compatibility and high photostability
Product Details
Penta·His Alexa Fluor 488 Conjugate and Penta·His Alexa Fluor 647 Conjugate (His tag Alexa Fluor conjugates) are for sensitive, direct immunofluorescent detection of recombinant proteins carrying His tags (consisting of at least five consecutive His residues) at 488 and 647 nm. Penta·His Alexa Fluor Conjugates are used for applications including immunocytochemistry, protein localization and targeting studies and fluorescence-activated cell sorting (FACS).
Performance
| Features | Penta·His Alexa Fluor 488 Conjugate | Penta·His Alexa Fluor 647 Conjugate |
|---|---|---|
| Detection | Five consecutive histidine residues | Five consecutive histidine residues |
| Antibody Isotype | Mouse IgG1 | Mouse IgG1 |
| Cross-reactivity | Negligible with crude E. coli, yeast, mammalian or insect cell lysates | Negligible with crude E. coli, yeast, mammalian or insect cell lysates |
| Form | Stabilized stock solution | Stabilized stock solution |
| Working solution | Depends on application | Depends on application |
Principle
Penta·His Antibodies are available as Alexa Fluor 488 and 647 conjugates (His tag Fluor 647, His tag Fluor 488), giving a range of highly specific reagents whose emission wavelengths cover a wide portion of the visible spectrum for Penta·His immunofluorescent detection.
Procedure
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Applications
The Penta·His Alexa Fluor 488 Conjugate and Penta·His Alexa Fluor 647 Conjugate can be used in immunofluorescence procedures such as flow cytometry, confocal laser microscopy, immunocytochemistry, immunohistochemistry, protein localization and targeting studies.
| Features | Specifications |
|---|---|
| Detection | Direct |
| Epitope detected | HHHHH |
| Tag | His tag |
Resources
Safety Data Sheets (1)
Kit Handbooks (1)
Certificates of Analysis (1)
Publications
Cleavage of the plasma membrane Na+/Ca2+ exchanger in excitotoxicity.
Cell; 2005; 120 (2):275-85 2005 Jan 28 PMID:15680332