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Ni-NTA HisSorb Plates (5)
Cat. No. / ID: 35061
5 Ni-NTA-coated, transparent 96-well plates
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Ni-NTA HisSorb Plates are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Enhanced signal-to-noise ratio and reproducibility
- Convenient multiwell-based assay system
- Reduced optimization for faster assay development
Product Details
Ni-NTA HisSorb Plates (Ni-NTA assay plates) are 96-well plates whose wells are coated with Ni-NTA. They are used for immobilization of His-tagged proteins in protein interaction assays, such as ELISA.
Principle
Ni-NTA HisSorb Plates provide the power of the QIAexpress Ni-NTA System in solid-phase 96-well format for convenient assay and screening of His-tagged biomolecules, even if crude lysates. Ni-NTA moieties, which coat the entire surface of each well, deliver effective, directed immobilization of functionally active 6xHis-tagged molecules, leading to more reliable and reproducible assay results and higher signal-to-noise ratio than are possible with other systems.
Procedure
TheQIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Applications
Ni-NTA HisSorb Plates are suitable for a range of applications, including:
- Quantitation of His-tagged molecules
- ELISA
- Hybridoma and antibody screening
- Drug discovery
- Screening expression clones and engineered enzymes
| Features | Specifications |
|---|---|
| Epitope detected | Histidine residued epitope |
| Format | Plate based |
| Instrument | Manual |
| Ligand on bead or plate | Ligand on plate |
| Tag | 6xHis tag |
Resources
Quick-Start Protocols (1)
Kit Handbooks (2)
Technical Information (1)
Safety Data Sheets (1)
Certificates of Analysis (1)