Uncover ultra-rare variants in cfDNA

Striking gold in your cfDNA samples – finding ultra-rare variants with confidence

Why is rare variant detection and discovery from cell-free DNA (cfDNA) so challenging? If you find yourself asking this question, you’re not alone. cfDNA is highly fragmented, unstable in biofluids and at low concentrations. Meanwhile, circulating-tumor DNA (ctDNA) only represents 1–2% of total cfDNA in non-advanced, non-metastatic cancers. It’s like looking for a needle in a haystack and requires highly sensitive and specific techniques. Sequencing errors, enrichment biases and false positives further add to the ever-expanding list of bottlenecks. Our QIAseq Targeted cfDNA Ultra Panels can help you overcome these obstacles, allowing you to detect ultra-rare variants down to 0.1% variant allele frequency (VAF) from cfDNA.