Single-cell studies in recent years have revealed that organisms are composed of thousands of unique and unrepeatable cell types. Conducting single-cell genomic analyses using next-generation sequencing (NGS) methods, similar to low-input sequencing, has traditionally been challenging due to the limited amount of genomic DNA present in single cells. This limitation becomes even more pronounced when the studied population is small, such as in the case of rare cells. High-fidelity (HiFi) and high-quality genomic DNA amplification is essential for single-cell sequencing, which is strongly dependent on the quality of the isolated cells. Therefore, the method used to isolate single cells becomes an important consideration for ensuring cell viability and nucleic acid integrity. Namocell’s patented gentle sorting and cell enrichment technology overcomes the aforementioned challenges to provide a higher quantity and quality of cell samples for sequencing. As a result, a complete and accurate picture of the studied samples can be achieved.

Whether you’re a beginner or expert in single-cell analysis, join this informative webinar to learn about the considerations, challenges and new technologies for low-input or rare single-cell genomics, followed by a discussion on the latest tips for low-input NGS.
About the speaker
Felix Alonso-Valenteen Ph.D., Field Application Scientist
Felix Alonso-Valenteen is a Field Application Scientist at Namocell and is responsible for customer success and application development based in the mid-Atlantic region of the United States. Felix received his Ph.D. in Biomedical Sciences and Translational Medicine from Cedars-Sinai Medical Center in Los Angeles, California, where he focused on novel nanoparticle therapeutics R&D and completed a postdoctoral fellowship at Cedars-Sinai.
Ioanna Andreou, Ph.D., Associate Director R&D, NGS Applications Development
Ioanna Andreou is an Associate Director at QIAGEN R&D and is responsible for developing technologies for NGS applications covering single-cell and DNA methylation analysis.She received her Ph.D. from the University of Freiburg, focusing on early cancer detection based on autoimmune response. She joined QIAGEN in 2002 after a postdoctoral fellowship at the University of Cologne.
Date of recording:Thursday, April 28, 2022
Duration:60 minutes
Core Lab / Reference Lab
Single Cell Analysis
Next Generation Sequencing