The responsibility of fertility is disproportionately placed on women, who bear most of the health, emotional and financial burdens. Infertility is rapidly rising, with one in six Australian couples seeking assisted reproductive technologies to help them start a family, and over 50% of cases involving a male factor. Despite these contributions, we still don’t fully understand the mechanisms that create competent sperm for fertilization, which occurs during their transit from the epididymis, a highly specialized region of the male reproductive tract. 

To address this knowledge gap, we used high-resolution tandem mass spectrometry and in-silico analyses to characterize proteome-wide changes in mouse sperm. We studied changes associated with their transit through the epididymis and elicited in response to capacitation stimuli, such as maturation initiated upon contact with the female reproductive tract. 

In this webinar, you’ll learn about:

  • The unprecedented depth of >6000 proteins and 14,586 site-specific phosphorylation events across these sperm populations that we discovered using QIAGEN Ingenuity Pathway Analysis (IPA) 
  • How sperm shed over 56% of their proteins during this process, producing a refined fertilization-competent cell
  • How this reduced proteomic complexity of sperm coincides with the activation of certain functions, such as sperm motility and capacitation
  • Key proteins and kinases we identified through selective pharmacological inhibition that regulate sperm function 
  • The role of RHOA on mature spermatozoa to reduce the rate of acrosome reaction by about 40%

Our data represent an essential paradigm shift in our understanding of male fertility regulation. They will contribute to important advancements in developing non-hormonal male contraceptives and robust sperm selection biomarkers for infertile men.

About the speaker
Dr. David Skerrett-Byrne, Postdoctoral Research Fellow, Infertility and Reproduction Research Program HMRI
The University of Newcastle, Australia
Dr. David Skerrett-Byrne is an emerging, internationally recognized research leader in reproductive biology, multi-omics analyses and bioinformatics at The University of Newcastle and Hunter Medical Research Institute. While still early in his career, he has 33 published research articles across multidisciplinary top-tier journals such as Cell Reports and Molecular and Cellular Proteomics. His research has provided unique insights into the molecular mechanisms and proteomic remodeling driving functional sperm maturation and fertilization-competent sperm cells.Dr. Skerrett-Byrne’s successes have led to >$1.3 million in competitive national, industry and philanthropic grants and several invited presentations. In recognition of his research excellence and rising reputation, he was awarded the 2023 Ken Mitchelhill Young Investigator Award from the Australasian Proteomics Society and a Rod Rickards Fellowship from The Australian Academy of Science (’23), supporting research training and establishing collaborative links with Dr. Raffaele Teperino (Helmholtz Zentrum München, Germany).
Date of recording:Thursday, November 2, 2023
Duration:60 minutes
Biomedical Research
Bioinformatic - Analysis
Informatics & Data