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PyroMark Q24 System

Cat. No. / ID: 9001514

Instrument and software for Pyrosequencing analysis: includes installation, training, and 1-year warranty on parts and labor

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Protocol Files (9)
Cleanup of bisulfite converted DNA

For cleanup of DNA from bisulfite reactions using the EpiTect Bisulfite Kit.

Sample Size 140 µl bisulfite reaction
Elution volume 2 x 20 µl
Applications Cleanup, DNA
Starting material Bisulfite converted DNA
Purification of DNA from previously isolated genomic DNA V1

This protocol is for the purification of nucleic acids from any sample source containing challenging impurities, including humic acids, heme, polysaccharides, polyphenols, fluvic acids, lipids and dye using the DNeasy PowerClean Pro kit.


Sample Size 175 µl
Elution volume 50–100 µl in increments of 10 µl, default set to 100 µl
Applications Cleanup
Starting material Fluid samples
QIAquick PCR Purification protocol

This protocol is for purification of single- or double-stranded DNA fragments (100 bp to 10 kb) from amplification reactions using the QIAquick PCR Purification Kit.

Sample Size 5-100 µl PCR samples
Elution volume 30-100 µl in increments of 10 µl, default 50µl
Applications Cleanup, DNA
Starting material Amplification reactions
Cleanup of 100 ng or more bisulfite converted DNA

This protocol is for cleanup of up to 100 ng bisulfite converted DNA using the EpiTect Plus DNA Bisulfite Kit.

Sample Size 140 µl bisulfite reaction
Elution volume 10-16 µl in increments of 1 µl; default 16 µl
Applications Cleanup, DNA
Starting material Bisulfite converted DNA, Bisulfite converted DNA derived from FFPE tissues
Purification of DNA from previously isolated genomic DNA V1

This protocol is for the purification of nucleic acids from any sample source containing challenging impurities, including humic acids, heme, polysaccharides, polyphenols, fluvic acids, lipids and dye using the DNeasy PowerClean Pro kit. This protocol contains all inhibitor removal steps.


Sample Size 100 µl
Elution volume 50–100 µl in increments of 10 µl, default set to 100 µl
Applications Cleanup
Starting material Fluid samples
Cleanup of bisulfite converted DNA from FFPE

For cleanup of DNA (from FFPE tissues) from bisulfite reactions using the EpiTect Bisulfite Kit

Sample Size 140 µl bisulfite reaction
Elution volume 2 x 20 µl
Applications Cleanup, DNA
Starting material Bisulfite converted DNA derived from FFPE tissues
Cleanup of 100 ng or more bisulfite converted DNA

This protocol is for cleanup of 100 ng or more bisulfite converted DNA using the EpiTect Plus DNA Bisulfite Kit.

Sample Size 140 µl bisulfite reaction
Elution volume 10-16 µl in increments of 1 µl; default 16 µl
Applications Cleanup, DNA
Starting material Bisulfite converted DNA, Bisulfite converted DNA derived from FFPE tissues
Purification of PCR products from 100-200 µl PCR samples

This protocol is for purification of double-stranded DNA fragments (100 bp to 10 kb) from PCR reactions of up to 200 µl sample volume (e.g., pooled samples) using the QIAquick PCR Purification Kit.

Sample Size 100-200 µl PCR samples
Elution volume 30-100 µl in increments of 10 µl, default 50µl
Applications Cleanup, DNA
Starting material Amplification reactions
Cleanup of 100 ng or more bisulfite converted DNA

This protocol is for cleanup of 100 ng or more bisulfite converted DNA using the EpiTect Fast DNA Bisulfite Kit.

Sample Size 140 µl bisulfite reaction
Elution volume 10-16 µl in increments of 1 µl; default 16 µl
Applications Cleanup, DNA
Starting material Bisulfite converted DNA

Publications

Genetic diagnostics of functional variants of the human dopamine D2 receptor gene.
Doehring A; Kirchhof A; Lötsch J;
Psychiatr Genet; 2009; 19 (5):259-68 2009 Oct PMID:19512960
Assessing combined methylation-sensitive high resolution melting and pyrosequencing for the analysis of heterogeneous DNA methylation.
Candiloro IL; Mikeska T; Dobrovic A;
Epigenetics; 2011; 6 (4):500-7 2011 Apr 1 PMID:21364322
Cell specific patterns of methylation in the human placenta.
Grigoriu A; Ferreira JC; Choufani S; Baczyk D; Kingdom J; Weksberg R;
Epigenetics; 2011; 6 (3):368-79 2011 Mar 1 PMID:21131778
CpG island hypermethylation of the neurofibromatosis type 2 (NF2) gene is rare in sporadic vestibular schwannomas.
Kullar PJ; Pearson DM; Malley DS; Collins VP; Ichimura K;
Neuropathol Appl Neurobiol; 2010; 36 (6):505-14 2010 Oct PMID:20831745
Systematic cross-validation of 454 sequencing and pyrosequencing for the exact quantification of DNA methylation patterns with single CpG resolution.
Potapova A; Albat C; Hasemeier B; Haeussler K; Lamprecht S; Suerbaum S; Kreipe H; Lehmann U;
BMC Biotechnol; 2011; 11 :6 2011 Jan 14 PMID:21235780