RNeasy 96 QIAcube HT Kit

動物およびヒトの細胞/組織からのハイスループットRNA自動分離

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RNeasy 96 QIAcube HT Kit (5)

Cat. No. / ID:  74171

For 480 preps: RNeasy 96 plates, RNase-free water, buffers
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KitAccessories
RNeasy 96 QIAcube HT Kit
QIAcube HT Plasticware
RNeasy 96 QIAcube HT Kitは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 簡便で信頼できる自動化精製により費用と時間の節約が可能
  • トータルRNAまたはmiRNAを含むトータルRNA分離のためのフレキシブルな操作
  • 各種スタートサンプルおよび目的のサイズ分布 用プロトコール
  • ダウンストリームアプリケーションに即使用可能な高品質RNA
  • 少量のスタートサンプルで一定したRNA収量

Product Details


RNeasy 96 QIAcube HT KitはQIAcube HTシステムを用いた動物およびヒトの細胞/組織サンプルからのトータルRNAまたはmiRNAを含むトータルRNAの簡便な自動化精製を実現します。便利な96ウェルフォーマットで、実績のあるRNeasyのシリカメンブレンテクノロジーを用いることにより、夾雑物および阻害物が除去されたダウンストリーム解析に即使用可能な高品質の病原体核酸が得られます。

Performance

96ウェルプレート内のウェル間で有意な変動のない、直線性のあるサンプル回収を実現します(図  トータルRNAの直線性のある回収 および  再現性ある96ウェルでのRNA精製)。

この経済的で使いやすいシステムにより、信頼性の高い他のQIAGEN精製テクノロジーと同様の結果が得られます(図  組織からRNAの自動精製)。QIAxcel Advancedシステムを用いて行なった、QIAcube HTで精製されたサンプルの解析は、高いRNA Integrity Score(RIS)を示し、他のQIAGENの自動化ソリューションと同等の結果を示します(図  組織から精製した高品質なRNA)。

RNeasy 96 QIAcube HT Kitは少量のスタートサンプルから一定の収量を実現します(表 RNeasy 96 QIAcube HT Kitを用いた細胞からの代表的な トータルRNA収量)。

RNeasy 96 QIAcube HT Kitでの細胞からの代表的なトータルRNA収量
細胞株  由来 RNA の収量
(µg per 105 cells)*
 HeLa  ヒト子宮頸がん 1.6
 LMH  トリ肝がん 1.3
 COS-7  サルの腎臓(SV-40形質転換) 3.1 
 Huh7  ヒト肝がん 2.0 
 Jurkat  ヒトT細胞白血病 1.4 
 K-562  急性転化ヒト慢性骨髄性白血病 1.9 
* 種、発生段階、および生長環境などの要因によって量は変化します。RNeasy調製法で200ヌクレオチド以上のRNAを精製した場合、全細胞性RNAの15~20%を形成する5S rRNA、tRNA、およびその他の分子量の低いRNAは回収されたトータルRNAには含まれません。

 

See figures

Principle

RNeasy 96 QIAcube HT KitはQIAcube HT装置上で5 x 105の動物/ヒト細胞、または40 mg以下の組織からのトータルRNAまたはmiRNAを含むトータルRNAの自動化精製を実現します。スタートマテリアルおよび精製RNAのサイズ分布によって、6つのプロトコールからお選びいただけます。細胞サンプルはRNeasy 96 QIAcube HT Kitと専用のキットプロトコールを用いてすぐに精製することができます。組織サンプル(40 mg以下)およびsmall RNAの精製用サンプルは、QIAzol Lysis Reagentでサンプル溶解後に処理されます。この操作で得られた高品質RNAはダウンストリームアプリケーションでも良好な結果を示します。

RNeasy 96 QIAcube HT Kitはシリカベースのメンブレンへの選択的結合特性と96ウェルフォーマットでのハイスループット処理を組み合わせ、QIAcube HT装置上で24~96サンプルの同時完全自動化用にデザインされています。
 
細胞からのトータルRNA精製に関する仕様
仕様 概要
サンプル数 24~96サンプル(8サンプル単位で処理)
スタートサンプル量 140 μl のBuffer RLTでホモジナイズされた細胞、最大5 x 105 
溶出量 110 μl
所要時間 約93 または114* 分で96サンプル
約54 または73* 分で24サンプル
* 適切なDNase処理を含む。

組織からのトータルRNA精製に関する仕様
仕様 概要
サンプル数 24~96サンプル(8サンプル単位で処理)
スタートサンプル量 750 μlのQIAzol Lysis Reagent中でホモジナイズした、40 mgの凍結組織または20 mgの固定化された組織から得られた350 μlの水相
溶出量 110 μl
所要時間 約73 または93* 分で96サンプル
約34 または54* 分で24サンプル
* 適切なDNase処理を含む。

細胞および組織からのmiRNAを含むトータルRNA精製に関する仕様
仕様 概要
サンプル数 24~96サンプル(8サンプル単位で処理)
スタートサンプル量 750 μlのQIAzol Lysis Reagent中でホモジナイズした、40 mgの凍結組織または20 mgの固定化された組織から得られた350 μlの水相
溶出量 110 μl
所要時間 約78分で96サンプル
約38分で24サンプル

Procedure

マニュアル操作で行なう細胞の溶解は、グアニジンチオシアネートを含む非常に強い変性条件によりRNaseが直ちに不活性化され、インタクトなRNAの精製を実現します。ランがスタートすると、適切な結合条件にするためにライセートにエタノールが添加され、サンプルはRNeasy 96 plateの各ウェルにアプライされます。トータルRNAは結合し、夾雑物は効果的に洗浄除去されます。RNaseフリー水を用いて少量で溶出された高品質RNAはどのようなダウンストリームアプリケーションにも即使用可能です。

動物またはヒト細胞からのトータルRNAの自動化精製では200ヌクレオチド以上の全てのRNA分子が精製されます。ほとんどの200ヌクレオチド未満のRNA(トータルRNAの15~20 %を占める5.8S rRNA、5S rRNA、tRNAなど)は選択的に排除されるので、mRNAが高濃度で得られます。精製したRNAのサイズ分布は、small RNAが効率的に沈殿しないCsCl密度勾配遠心法に匹敵します。

組織からのトータルRNA精製、または細胞および組織からのsmall RNAを含むトータルRNAの精製には溶解バッファーとしてQIAzol Lysis Reagentのご使用をお勧めします。操作手順の詳細についてはキットのハンドブックをご覧ください。

Applications

RNeasy 96 QIAcube HT Kitは、ドラッグスクリーニングおよび基礎研究分野などでの研究に使用できる、効果的でハイスループットのRNA精製を提供します。精製されたRNAは以下のようなダウンストリームアプリケーションに使用可能です。
  • RT-PCR およびqRT-PCR 
  • ディファレンシャルディスプレイ
  • cDNA合成
  • ノーザン、ドット、スロットブロット解析
  • プライマーエクステンション
  • Poly A+ RNA 調製
  • RNaseおよびS1 nuclease protection

Supporting data and figures

Resources

プロトコール (12)
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Q Protocol notes for purification of total RNA from animal or human cells, including DNase digestion, on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the RNeasy 96 QIAcube HT total RNA Cell with DNase V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software, For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Q Protocol notes for purification of total RNA from animal or human cells on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the RNeasy 96 QIAcube HT total RNA Cell V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Q Protocol notes for automatic transfer of upper aqueous phase to an empty S-Block on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the RNeasy 96 QIAcube HT pre-treatment V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Q Protocol notes for purification of total RNA from tissue on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the RNeasy 96 QIAcube HT total RNA Tissue V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Q Protocol notes for purification of total RNA or total RNA, including miRNA, from animal tissue and cells on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the miRNeasy 96 QIAcube HT V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Q Protocol notes for purification of total RNA from tissue, including DNase digestion, on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the RNeasy 96 QIAcube HT total RNA Tissue with DNase V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
MSDS (1)
Download Safety Data Sheets for QIAGEN product components.
テクニカルインフォメーション (2)
クイックスタートプロトコール (4)
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
For use with the RNeasy 96 QIAcube HT Kit and QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
キットハンドブック (2)
Handbook for purification of total RNA, or total RNA, including miRNA, from animal tissue and cells on the QIAcube HT using the RNeasy 96 QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software.
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
機器ユーザーマニュアル (3)
For use with the QIAcube HT Prep Manager Software
For QIAcube HT and QIAxtractor
For QIAcube HT and QIAxtractor
パンフレット (1)
For fast and reliable automated 96-well nucleic acid purification
Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (1)
For fast and reliable automated 96-well nucleic acid purification
Kit Handbooks (2)
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
Handbook for purification of total RNA, or total RNA, including miRNA, from animal tissue and cells on the QIAcube HT using the RNeasy 96 QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software.
Quick-Start Protocols (4)
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
For use with the RNeasy 96 QIAcube HT Kit and QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
Protocol Files (12)
Q Protocol notes for purification of total RNA or total RNA, including miRNA, from animal tissue and cells on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the miRNeasy 96 QIAcube HT V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Q Protocol notes for automatic transfer of upper aqueous phase to an empty S-Block on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the RNeasy 96 QIAcube HT pre-treatment V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software, For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Q Protocol notes for purification of total RNA from animal or human cells, including DNase digestion, on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the RNeasy 96 QIAcube HT total RNA Cell with DNase V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Q Protocol notes for purification of total RNA from animal or human cells on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the RNeasy 96 QIAcube HT total RNA Cell V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Q Protocol notes for purification of total RNA from tissue, including DNase digestion, on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the RNeasy 96 QIAcube HT total RNA Tissue with DNase V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Q Protocol notes for purification of total RNA from tissue on the QIAcube HT using the RNeasy 96 QIAcube HT Kit; use with the RNeasy 96 QIAcube HT total RNA Tissue V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Instrument User Manuals (3)
For QIAcube HT and QIAxtractor
For use with the QIAcube HT Prep Manager Software
For QIAcube HT and QIAxtractor

FAQ

Can I run the QIAcube HT with the QIAxtractor software or vice versa?

No, the two software applications are not interchangeable.

 

Additionally, the QIAcube HT and QIAxtractor software cannot be installed on the same computer. The QIAcube HT software versions is v4.17 or higher, while the QIAxtractor software version is v4.16 or lower.

FAQ ID -9102
For what batch size is the QIAcube HT Plasticware designed?
The QIAcube HT Plasticware content is calculated for use with the extraction protocol working in batches of 96 samples. If running smaller batches and/ or the transfer protocol, additional consumables are required. 
FAQ ID - 4010
Can I upgrade a Corbett CAS-1820 to a QIAcube HT?

No, this upgrade is not possible.

FAQ ID -9104
What file is required for reporting a QIAcube HT or QIAxtractor issue?

The Support Packages are important when reporting an issue to QIAGEN Technical Services. Upon completion of each run, the software automatically saves a support package.

 

To retrieve a support package, click Help in the QIAcube HT or QIAxtractor software and select Choose support package. In the new windows, select the support package that corresponds to the issue and send it to QIAGEN Technical Services.

 

FAQ ID -9100
I am getting vacuum errors on my QIAcube HT. What should I do?

1. Check the vacuum tubing to make sure it is not pinched or obstructed in some way. Sometimes the tubing can become pinched against the bench top when the vacuum station is placed too far from the instrument.

Waste bottle tubing

2. Make sure that the channeling adapter is properly placed. Check if the carriage is moving the plate completely from the waste chamber position to the elution chamber position. If the calibration seems incorrect, the instrument may need to be re-taught at this position.

Adaptors

3. When using less than a full plate, make sure all the unused columns are covered with an adhesive seal.

Also, make sure each column of 8 samples is full. When processing a number of samples that results in < 8 samples per column, use water as a substitute in the empty positions until the column is filled. This ensures even vacuum across all unsealed well positions.

4. Make sure correct plates are used for the selected protocol.

5. When the vacuum is on, please listen for any hissing noise from the tubing that is coming down to the waste container. This could indicate leaks in the tubing.

If the issue persists, please contact QIAGEN Technical Service.



FAQ ID -9106
What do I do if the QIAcube HT or QIAxtractor software runs in virtual mode while the instrument is connected to the computer?

Make sure the QIAcube HT or QIAxtractor software uses the same COM port number as assigned to the QIAcube HT or QIAxtractor instrument on the computer. The COM port setting can be checked by clicking Options\Robot Setup\Select COM port in the QIAcube HT or QIAxtractor software.

 

Ensure that the cable between the instrument and the computer is securely inserted. Power on the instrument first, and then launch the QIAxtractor/QIAcube HT software. 

 

The instrument has both RS-232 (serial) and USB interfaces for computer connection. Try an RS-232 cable if the communication cannot be established between the instrument and computer with a USB cable.

 

If the issue persists, please contact QIAGEN Technical Services.

 

FAQ ID -9101