ライゲーション計算ツール

一般的なライゲーション反応に適した範囲で、目的のインサート/ベクターモル比にするために必要なインサート量を計算します。

icon_0330_cc_gen_calculator-sターゲットを計算する関心のあるターゲットのデータを入力し、シークエンシング計算を受け取る
洞察

 

インサートDNA所要量 [g] = ベクターDNA量 [g] x {インサートDNA長/ベクターDNA長} x 目的のインサート/ベクターモル比

注:モル比は{1, 2, 3, 5 ,7}から選択します。

インサート長はベクター長よりも小さい値にします。

もっと見る

最初に、インサートDNAとベクターDNAを制限エンドヌクレアーゼで消化し、粘着末端(または平滑末端)を持つDNA断片を生成します。次に、目的のDNA断片を切り取った後に結合し、DNAリガーゼで処理します。最後に、得られたインタクトなベクターをコンピテントなE. coli細胞に形質転換します。形質転換した細胞は適切な遺伝子選別方法によって識別します。 

 

DNAライゲーションプロセスの各ステップと一般的な反応について詳しく知りたいですか? 詳細はこちら

DNAライゲーションに使用できる酵素を見る

 

もっと多くの酵素をお探しですか?

さまざまな分子生物学アプリケーションのために設計された高品質な製品をご覧ください。

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